D-Dimer (+ Fibrinogen) Antikörper

Antigen: D-Dimer (+ Fibrinogen)

Klonalität: Monoklonal (DD-6)

Wirt: Maus

Applikation: Enzyme Immunoassay (EIA), ELISA (Detektionsantikörper), Western Blot (WB)

Produktnummer: ABIN191196

Produktbeschreibung

Immunogen: Hybridoma Clone s have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with homogenized fibrin clot, D-dimer or high molecular weight fibrin degradation products.

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: DD-6

Spezifität: All MAbs recognized D-dimer and high molecular weight fibrin degradation products with different pecificities. MAb DD93 recognizing a cross-linked region of D-Dimer. s DD1 DD2 DD3 DD22 DD41 DD44 DD46 DD93 No cross-reaction with fibrinogen and D-monomer DD4 DD5 DD6 Cross-reaction with fibrinogen, Low cross-reaction with D-monomer IgG1 for MAb DD93 IgG2a for MAbs DD1, DD6, DD22, DD41, and DD46 IgG2b for MAbs DD2, DD3, DD4, DD5 and DD44

Anwendungen

Applikationshinweise: D-dimer and high molecular weight fibrin degradation products immunoassay. All antibodies recognize D-dimer in ELISA. All MAbs recognize D-dimer in Western blotting under non-reducing conditions. MAbs DD22, DD41, DD44, DD46 interact with β-chain of D-dimer in Western blotting under reducing conditions. Recommended pairs to be used in a one-step sandwich immunoassay for D-dimer detection in human plasma (coating – conjugate): DD2 – DD41 and DD2 – DD44. Recommended pairs to be used in two-step sandwich immunoassay for D-dimer detection in human plasma (coating – detection)*: DD1 – DD6, DD1 – DD4, DD1 – DD5, DD3 – DD4, DD3 – DD6, DD2 – DD6, DD2 – DD4 and DD2 – DD5 *To be analyzed in a two-step sandwich immunoassay, plasma must be diluted with 10 mM Tris-HCl buffer, pH 7.5, containing 1 M NaCl and 0.1 % Tween 20. To avoid non-specific binding the final NaCl concentration in plasma samples must be 0.5 M or more.

Buffer: PBS, pH 7.4, 0.1 % sodium azide (NaN3) Material safety note: This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material. Contains sodium azide (0.1 %) as preservative

Lagerung: + 4 °C

Beschränkungen: Nur für Forschungszwecke einsetzbar