Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1) (Internal Region) Antikörper

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  • APPL
  • DIP13alpha
  • 2900057D21Rik
  • 7330406P05Rik
  • AI585782
  • AW209077
  • BB022931
  • C88264
  • DIP13
  • RGD1309388
  • adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
  • APPL1
  • Appl1
Epitop
Internal Region
14
9
5
5
4
3
3
2
2
2
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus), Hund, Rind (Kuh)
64
32
20
6
4
3
3
3
3
3
1
1
Wirt
Ziege
44
19
3
Klonalität
Polyklonal
Konjugat
Unkonjugiert
1
1
1
1
1
1
Methodentyp
Peptide ELISA
Applikation
ELISA, Western Blotting (WB)
60
32
17
12
3
3
1
1
1
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Immunogen Peptide with sequence C-NRYSRLSKKRENDK, from the internal region of the protein sequence according to NP_036228.1.
Blocking Peptide Zu diesem Produkt passt das Blocking Peptide ABIN369506.
Reinigung Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Andere Bezeichnung APPL1 (APPL1 Antibody Abstract)
Hintergrund Synonyms: APPL1, DIP13alpha, adaptor protein containing pH domain, PTB domain and leucine zipper motif, signaling adaptor protein DIP13alpha
Gen-ID 26060, 72993
NCBI Accession NP_036228
Applikationshinweise ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 80 kDa band observed in Rat Aortic Smooth Skeletal Muscle Cells (lane 1), intensity increased after infection with Human APPL1 adenovirus for 48h (lane 2). Calculated MW of 79.7 kDa according to NP_036228.1. The blot was co-labeled with Tubulin-specific antibodies as a loading control. Recommended concentration: 0.3-1 µg/mL.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Minimize freezing and thawing.
Lagerung -20 °C
Informationen zur Lagerung Aliquot and store at -20 °C.
Bilder des Herstellers
 image for anti-Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1) (Internal Region) Antikörper (ABIN190879) ABIN190879 (0.5µg/ml) staining of 1) Rat Aortic Smooth Muscle cell lysate 2) after in...
Allgemeine Veröffentlichungen Mao, Kikani, Riojas, Langlais, Wang, Ramos, Fang, Christ-Roberts, Hong, Kim, Liu, Dong: "APPL1 binds to adiponectin receptors and mediates adiponectin signalling and function." in: Nature cell biology, Vol. 8, Issue 5, pp. 516-23, 2006 (PubMed).