Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (Internal Region) Antikörper

Details zu Produkt Nr. ABIN190755, Anbieter: Anmelden zum Anzeigen
Antigen
  • p40phox
  • AI451400
  • NCF
  • P40PHOX
  • SH3PXD4
  • p40phox protein
  • neutrophil cytosolic factor 4
  • neutrophil cytosolic factor 4, 40kDa
  • p40phox
  • LOC100136241
  • Ncf4
  • NCF4
Epitop
Internal Region
19
9
8
4
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Human
117
58
44
7
7
5
4
4
3
3
Wirt
Ziege
91
19
11
Klonalität
Polyklonal
Konjugat
Unkonjugiert
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Methodentyp
Peptide ELISA
Applikation
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
74
40
32
26
23
5
4
3
3
2
2
1
Optionen
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Immunogen Peptide with sequence C-KDFPEEDDPTN, from the internal region of the protein sequence according to NP_000622.2, NP_038202.1.
Blocking Peptide Zu diesem Produkt passt das Blocking Peptide ABIN369246.
Spezifität This antibody is expected to recognise both reported isoforms (NP_000622.2 and NP_038202.1).
Kreuzreaktivität Hund
Reinigung Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Andere Bezeichnung NCF4 / P40PHOX (NCF4 Antibody Abstract)
Hintergrund Synonyms: NCF4, P40PHOX, neutrophil cytosolic factor 4, 40kDa, CTA-833B7.1, MGC3810, NCF, SH3PXD4, OTTHUMP00000028736, OTTHUMP00000043756, neutrophil NADPH oxidase factor 4, neutrophil cytosol factor 4, neutrophil cytosolic factor 4 (40kD)
Gen-ID 4689
NCBI Accession NP_038202, NP_000622
Forschungsgebiet Autophagy
Applikationshinweise ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx. 38 kDa band observed in lysates of cell line Daudi (calculated MW of 39.0 kDa according to NP_000622.2 and 38.8 kDa according to NP_038202.1). In transfected HEK293 transiently expressing P40PHOX a band of approx. 42 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 µg/mL.
IHC: In paraffin embedded Human Tonsil shows staining of the cytoplasm in some cells. Recommended concentration: 2-4 µg/mL.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Minimize freezing and thawing.
Lagerung -20 °C
Informationen zur Lagerung Aliquot and store at -20 °C.
Bilder des Herstellers
 image for anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (Internal Region) Antikörper (ABIN190755) ABIN190755 (0.5µg/ml) staining of Daudi cell lysate (35µg protein in RIPA buffer). Pr...
Allgemeine Veröffentlichungen Rioux, Xavier, Taylor, Silverberg, Goyette, Huett, Green, Kuballa, Barmada, Datta, Shugart, Griffiths, Targan, Ippoliti, Bernard, Mei, Nicolae, Regueiro, Schumm, Steinhart, Rotter, Duerr, Cho, Daly et al.: "Genome-wide association study identifies new susceptibility loci for Crohn disease and implicates autophagy in disease pathogenesis. ..." in: Nature genetics, Vol. 39, Issue 5, pp. 596-604, 2007 (PubMed).