Melan A (MLANA) Antikörper

Antigen: Melan A (MLANA)

Synonyme: MART1, MART-1

Klonalität: Monoklonal (EP1422Y)

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Western Blot (WB)

Produktnummer: ABIN189510

Produktbeschreibung

Weitere Bezeichnung: MART-1

Gen-ID: 2315, 77836, 293890

Swiss-Prot: Q16655

Isotyp: IgG  (Passende Sekundärantikörper)

Klon: EP1422Y

Beschreibung: MART-1 has been widely studied as a melanoma-specific antigen and as amelanosome-specific marker. A role for MART-1 in pigmentation is expected becauseits expression pattern and subcellular distribution is quite similar to the othermelanosomal proteins and usually correlates with melanin content. MART-1 forms acomplex with Pmel17 and affects its expression, stability, trafficking, and the processingwhich is required for melanosome structure and maturation. MART-1 is indispensablefor Pmel17 function and thus plays an important role in regulating mammalianpigmentation (1). A gene has been identified that encodes a melanocyte lineage-specificprotein (MART-1, melanoma antigen recognized by T cells 1) that is a widely sharedmelanoma antigen recognized by the T lymphocytes of patients with establishedmalignancy. Identification of this gene opens possibilities for the development ofimmunotherapies for patients with melanoma (2). Alternate Names: anti-Antigen LB39 AA antibody, anti-Antigen SK29 AA antibody, anti-MART 1 antibody,anti-MART1 antibody, anti-Melan A antibody, anti-Melan A protein antibody,anti-Melanoma antigen recognized by T cells 1 antibody, anti-MLAN A antibody,anti-MLANA antibody.

Spezifität: A synthetic peptide corresponding to residues on the N-terminus of human MART-1 wasused as an immungen. Species Reactivity: Cross reacts in Human. Does not react in Mouse or Rat.

Anwendungen

Applikationshinweise: Suggested working dilutions: immunohistochemistry 1:100-250,Western Blot 1:1000

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. Preservative: 0.01% sodium azide.

Lagerung: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Forschungsgebiet: Krebs

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis on melanoma lysate using anti-MART-1 RabMAb (cat. # 110-57185), dilution 1:1000.

Immunohistochemical staining of paraffin-embedded melanoma using anti-MART-1 RabMAb (cat. #110-57185).

Publikationen

Publikationen: Kawakami, Eliyahu, Delgado et al.: "Cloning of the gene coding for a shared human melanoma antigen recognized by autologous T cells infiltrating into tumor." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 9, pp. 3515-9, 1994 (PubMed).

Hoashi, Watabe, Muller et al.: "MART-1 is required for the function of the melanosomal matrix protein PMEL17/GP100 and the maturation of melanosomes." in: The Journal of biological chemistry, Vol. 280, Issue 14, pp. 14006-16, 2005 (PubMed).

Massoumi, Kuphal, Hellerbrand et al.: "Down-regulation of CYLD expression by Snail promotes tumor progression in malignant melanoma." in: The Journal of experimental medicine, Vol. 206, Issue 1, pp. 221-32, 2009 (PubMed).