Huntingtin (HTT) Antikörper

Antigen: Huntingtin (HTT)

Synonyme: HD, IT15, Hd, Hdh, AI256365, C430023I11Rik, hd, ZHD, HTT, HsapHD, dhtt, DmelCG9995, CG9995, Htt, HDH, SLC6A4, it15, MGC79590, htt, LOC100216476

Klonalität: Monoklonal (EP867Y)

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Durchflusszytometrie (FACS), Immunfluoreszenz (IF), Western Blot (WB)

Produktnummer: ABIN189434

Produktbeschreibung

Weitere Bezeichnung: Huntingtin

Gen-ID: 3064, 15194, 29424

Swiss-Prot: P42858

Isotyp: IgG  (Passende Sekundärantikörper)

Klon: EP867Y

Beschreibung: Huntingtin protein (Htt) is a 348 kDa protein product of Huntington's disease (HD, IT15)gene. Huntington's disease is a neurodegenerative disorder caused by a mutation onthe HD gene, producing a polyglutamin (polyQ) expansion on the N-terminus of Htt. Themutant Htt leads to cytotoxicity in the striatal neuron (1). This cytotoxicity is modulatedby proteolytic cleavage with caspases and calpains, producing N-terminal polyQfragments. A phosphorylation of Htt may also regulate the cleavage and cytotoxicity ofmutant Htt (2). The wild-type Htt up-regulates transcription of brain-derived neurotrophicfactor (BDNF), a pro-survival factor for striatal neurons. This suggests restoringwild-type Htt level and increasing BDNF expression level can be therapeutic treatmentfor Huntington's disease (3). Huntingtin is also cleaved by apopain, a cysteine proteaseproduct of death-gene for human, suggesting HD might be a disorder of inappropriateapoptosis (4). Alternate Names: anti-HD protein antibody, anti-HDH antibody, anti-HTT antibody, anti-HUNTINGTONCHOREA antibody, anti-Huntington disease protein antibody, anti-Huntington's diseaseprotein homolog antibody, anti-IT 15 antibody, anti-IT15 antibody, anti-ZHD antibody.

Spezifität: A synthetic peptide corresponding to residues specific to the apopain cleavage site ofhuman huntingtin protein was used as an immunogen. Species Reactivity: Cross reacts with Human, Mouse and Rat.

Anwendungen

Applikationshinweise: Suggested working dilutions: flow cytometry / FACS analysis 1:30,immunohistochemistry 1:250 to 500,Western Blot 1:10000,Immunocytochemistry 1:50 to 100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. Preservative: 0.01% sodium azide.

Lagerung: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Forschungsgebiet: Neurologie, Virologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis on SH-SY-5Y cell lysate using anti-huntingtin RabMAb (cat. # 110-57069), dilution 1:10,000.

Immunohistochemical analysis of paraffin-embedded human brain tissue using RabMAb. (cat. # 110-57069).