»
Antikörper
»
anti-Heat Shock Protein 70 (HSP70) (HSPA4) (C-Term) Antikörper
Heat Shock Protein 70 (HSP70) (HSPA4) (C-Term) Antikörper
| Antigen |
|
|
Epitope
|
|
| Klonalität |
Monoklonal (EP1007Y) |
|
Wirt
|
|
|
Reaktivität
|
Human (77), Maus (38), Ratte (Rattus) (36), Fisch (19), Fruchtfliege (Drosophila melanogaster) (19), Rind (Kuh) (17), Affe (17), Hund (16), Hamster (15), Kaninchen (15), Huhn (14), Meerschweinchen (13), Schaf (13), Schwein (12), Amphibien (10), Vogel (7), Hefe (Saccharomyces Cerevisiae) (6), Caenorhabditis elegans (C. elegans) (5), Yeast (Pichia pastoris) (5), Beluga (3), Xenopus laevis (2), Katze (1), Koralle (1), Ziege (1), Säugetiere (1), Pflanzen (1), Primate (1)
|
|
Applikation
|
 Alternativen Immunhistochemie (Gefrierschnitte) (IHC (fro)), Durchflusszytometrie (FACS), Immunpräzipitation (IP), Western Blot (WB)
Western Blot (WB) (68), Immunhistochemie (Paraffinschnitte) (IHC (p)) (32), Enzyme Immunoassay (EIA) (28), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (28), Immunfluoreszenz (IF) (27), Immunpräzipitation (IP) (26), Durchflusszytometrie (FACS) (11), Dot Blot (Dot) (3), Immunelektronenmikroskopie (EM) (3), ELISA (2), Radioimmunoassay (RIA) (1)
|
| Produktnummer |
ABIN189429 |
| Menge |
0.1 ml |
| Preis |
400,00 € Zzgl. Versandkosten €40,00 und MWSt
|
| Lieferung nach |
|
| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
|
Weitere Bezeichnung
|
Heat shock protein 70 / HSP70 (C-term)
|
|
Swiss-Prot
|
PO8107
|
|
Isotyp
|
IgG (Passende Sekundärantikörper)
|
|
Klon
|
EP1007Y
|
|
Beschreibung
|
The 70-kDa Heat Shock Protein (Hsp70) is a highly conserved member of the molecularchaperone network commonly found in nuclear, cytosolic, mitochondrial andendoplasmic reticulum compartments of all eukaryotic and prokaryotic cells. Hsp70 usesATP binding and hydrolysis to assist in efficient folding of both newly created andstress-denatured polypeptides. (1). Under environmental stress conditions, synthesis ofHsp70 is quickly up-regulated and redistributed throughout the cell. (2). It has beenshown that Hsp70 has the ability to bind with high affinity to the plasma membrane,activate NF-KappaB and also up-regulate expression of pro-inflammatory cytokinestumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta and IL-6 in human monocytes(3).
|
|
Spezifität
|
A synthetic peptide corresponding to residues near the C-term of human Hsp70 wasused as an immunogen. Species Reactivity: Cross reacts with Human. Does not react with Mouse or Rat.
|
|
Applikationshinweise
|
Suggested working dilutions: flow cytometry / FACS analysis 1:10,immunohistochemistry 1:50 to 100,immunoprecipitation 1:50,Western Blot 1:20000
|
|
Buffer
|
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. Preservative: 0.01% sodium azide.
|
|
Lagerung
|
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
|
|
Beschränkungen
|
Nur für Forschungszwecke einsetzbar
|
Alternativen zu Antigen "Heat Shock Protein 70 (HSP70) (HSPA4)", Typ "Antikörper" finden
|
Wirte
|
Maus (49), Kaninchen (29)
|
|
Reaktivitäten
|
Human (77), Maus (38), Ratte (Rattus) (36), Fisch (19), Fruchtfliege (Drosophila melanogaster) (19), Rind (Kuh) (17), Affe (17), Hund (16), Hamster (15), Kaninchen (15), Huhn (14), Meerschweinchen (13), Schaf (13), Schwein (12), Amphibien (10), Vogel (7), Hefe (Saccharomyces Cerevisiae) (6), Caenorhabditis elegans (C. elegans) (5), Yeast (Pichia pastoris) (5), Beluga (3), Xenopus laevis (2), Katze (1), Koralle (1), Ziege (1), Säugetiere (1), Pflanzen (1), Primate (1)
|
|
Applikationen
|
Western Blot (WB) (68), Immunhistochemie (Paraffinschnitte) (IHC (p)) (32), Enzyme Immunoassay (EIA) (28), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (28), Immunfluoreszenz (IF) (27), Immunpräzipitation (IP) (26), Durchflusszytometrie (FACS) (11), Dot Blot (Dot) (3), Immunelektronenmikroskopie (EM) (3), ELISA (2), Radioimmunoassay (RIA) (1)
|
|
Konjugate
|
FITC (3)
|
|
Epitope
|
AA 574-591 (3), C-Term (1), Center (1), pTyr41 (1), pTyr525 (1)
|
