HRAS (C-Term) Antikörper

Antigen: HRAS

Epitope: C-Term

Klonalität: Monoklonal (Y132)

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Immunfluoreszenz (IF), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN189394

Produktbeschreibung

Weitere Bezeichnung: HRAS (C-term)

Gen-ID: 3265, 15461, 293621

Swiss-Prot: P01112

Immunogen: A synthetic peptide corresponding to residues in the C-terminus of human H-Ras.

Isotyp: IgG  (Passende Sekundärantikörper)

Klon: Y132

Beschreibung: Ras, a signal transducer, was first characterized as the transforming genes of Harvey &Kristen sarcoma virus (1). The Ras family (H-Ras, N-Ras, and K-Ras) regulates cellgrowth, differentiation and apoptosis (2). Switching from an active or resting state, Rascan either bind GTP or GDP respectively. In the triphosphate conformation, Ras willinteract with GTPase activating protein (GAP) to increase its activity (3). Mutations inany of the three isoforms can convert these proteins into active oncogenes. Additionally,Ras mutations are found in 30 % of all human cancer (4). Alternate Names: anti-c H ras 1 antibody, anti-c H ras 1 protein antibody, anti-c H ras antibody, anti-chas/bas p21 protein antibody, anti-c ras Ki 2 protein antibody, anti-cHras antibody,anti-G1III6 N ras antibody, anti-GTPase HRas antibody, anti-H Ras 1 antibody, anti-Hras antibody, anti-H RasIDX antibody, anti-Ha ras antibody, anti-Harvey ras antibody,anti-Harvey rat sarcoma virus oncogene 1 antibody, anti-Hras 1 antibody, anti-HRASantibody, anti-Hras1 antibody, anti-K ras antibody, anti-N ras antibody, anti-N rasoncogene antibody, anti-P19 H RasIDX protein antibody, anti-p21 protein antibody,anti-p21ras antibody, anti-RASH 1 antibody, anti-RASH1 antibody, anti-Transformingprotein p21 antibody, anti-v Ha ras Harvey rat sarcoma viral oncogene homologantibody.

Spezifität: A synthetic peptide corresponding to residues in the C-terminus of human H-Ras wasused as an immunogen. The antibody does not cross-react with other Ras familymember. Species Reactivity: Reacts in human, mouse, and rat. Not tested in other species.

Anwendungen

Applikationshinweise: WB: 1:500. ICC: 1:100-250. IP: 1:50

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. Preservative: 0.01% sodium azide.

Lagerung: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis on A: MCF7 B: PC12 cell lysate using anti-H-Ras (C-term) RabMAb (catalog #110-57027), dilution 1:500

Immunohistochemical staining of MCF7 cells using anti-H-Ras (C-term) RabMAb (catalog #110-57027).