Keratin 6 (KRT6) Antikörper

Antigen: Keratin 6 (KRT6)

Synonyme: Krt2-6, Krt-2.6

Klonalität: Monoklonal (EPR1603Y)

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunfluoreszenz (IF), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN189291

Produktbeschreibung

Weitere Bezeichnung: Cytokeratin 6

Gen-ID: 3853, 683313

Swiss-Prot: P02538

Immunogen: Synthetic peptide corresponding to residues on the C-terminus of human CK-6A.

Isotyp: IgG  (Passende Sekundärantikörper)

Klon: EPR1603Y

Beschreibung: The human type II Cytokeratin 6 (CK6, 56 kDa) is expressed in a heterogeneous arrayof epithelial tissues under normal conditions, but is better known for its strong inductionin stratified epithelia that feature an enhanced cell proliferation rate or abnormaldifferentiation. It has been demonstrated that CK6 isoform, CK6a, is clearly thedominant CK6 isoform in skin tissue samples and cultured epithelial cell lines and thatthe various isoforms are differentially regulated within and between epithelial tissuetypes (1). The murine genome is known to have two keratin 6 (CK6) genes, mouse CK6,CK6a and MK6b. These genes display a complex expression pattern with constitutiveexpression in the epithelia of oral mucosa, hair follicles, and nail beds (2). Pachyony chiacongenital (PC) is characterized by hypertrophic nail dystrophy and associatedectodermal features. PC-1 subtype is associated with mutations in keratins 6a or 16,whereas PC-2 subtype is linked to mutations in keratins 6b or 17 (3).

Spezifität: A synthetic peptide corresponding to residues on the C-terminus of human CK-6A wasused as an immunogen. Species Reactivity: Reacts in Human. Does not react in Mouse or Rat. Not tested in other species.

Anwendungen

Applikationshinweise: WB: 1:10,000 IHC: 1:100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. Preservative: 0.01% sodium azide.

Lagerung: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Forschungsgebiet: Signaltransduktion, Zytoskelett, Extrazelluläre Matrix

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis on A431 lysate using anti-CK-6a RabMAb (cat. # 110-56917), dilution 1:100,000

Immunohistochemical staining of paraffin-embedded human squamous cervical carcinoma using anti-CK-6a RabMAb (cat. #110-56917).

Immunofluorescent staining of A431 cells using anti-CK 6A RabMAb (cat. #110-56917).