Cytokeratin 5 (KRT5) Antikörper

Antigen: Cytokeratin 5 (KRT5)

Synonyme: KRT79, CK5, KRT5, K5, Tfip8, Krt2-5, AW146334, 3300001P10Rik, DDD, EBS2, KRT5A

Klonalität: Monoklonal (EP1601Y)

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunfluoreszenz (IF), Western Blot (WB)

Produktnummer: ABIN189290

Produktbeschreibung

Weitere Bezeichnung: Cytokeratin 5

Gen-ID: 3852, 369017

Swiss-Prot: P13647

Immunogen: Synthetic peptide (Human).

Isotyp: IgG  (Passende Sekundärantikörper)

Klon: EP1601Y

Beschreibung: Keratins are a family of structurally related proteins that form the intermediate filamentcytoskeleton in epithelial cells. The 58-kD keratin CK-5 is highly similar to other type IIkeratins and less similar to type I keratins and other intermediate filament proteins. The58-kD keratin is regulated by retinoids in several tissues and is one of four keratinsabundantly expressed in epidermal keratinocytes, where it may be important inmaintaining structural integrity of the integument (1). Keratin 5 (CK-5) mRNA and proteinare shown to be expressed in normal mammary epithelial cells in culture and are absentfrom tumor-derived cell lines. This makes CK-5 an important marker in the tumorigenicprocess, distinguishing normal from tumor cells, and decreased CK-5 expressioncorrelates with tumorigenic progression (2). Dowling-Degos disease (DDD) is anautosomal dominant genodermatosis characterized by progressive and disfiguringreticulate hyperpigmentation of the flexures. Loss of function of CK-5 suggests a crucialrole for keratins in the organization of cell adhesion, melanosome uptake, organelletransport, and nuclear anchorage (3).

Spezifität: A synthetic peptide corresponding to residues on the C-terminus of human CK-5 wasused as an immunogen. Species Reactivity: Reacts in Human. Does not react in Mouse or Rat. Not tested in other species.

Anwendungen

Applikationshinweise: WB: 1:10,000 IHC: 1:100

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. Preservative: 0.01% sodium azide.

Lagerung: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Forschungsgebiet: Signaltransduktion, Zytoskelett, Zytokeratine, Extrazelluläre Matrix

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis on A431 cell lysate using anti-CK-5 RabMAb (cat. # 110-56916), dilution1:10,000

Immunohistochemical analysis of paraffin-embedded human transitional urinary bladder carcinoma using anti-CK5RabMAb.(cat.#110-56916).

Immunofluorescent staining of A431 cells using anti-CK-5 RabMAb (cat. #110-56916).