Calponin 1 (CNN1) Antikörper

Antigen: Calponin 1 (CNN1)

Synonyme: SMCC, Sm-Calp, CN, CnnI, cnn2, MGC69240, CNN1, MGC137768

Klonalität: Monoklonal (EP798Y)

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunfluoreszenz (IF), Western Blot (WB)

Produktnummer: ABIN189195

Produktbeschreibung

Weitere Bezeichnung: Calponin-1

Gen-ID: 1264, 12797, 65204

Swiss-Prot: P51911

Immunogen: Synthetic peptide corresponding to residues near the C-term of Calponin (Human).

Isotyp: IgG  (Passende Sekundärantikörper)

Klon: EP798Y

Beschreibung: Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-bindingprotein thought to be involved in regulation of actomyosin as well as the regulation ormodulation of contraction (1). Calponin binds filamentous actin (F-actin) through twodistinct sites ABS1 and ABS2, with an affinity in the low micromolar range (2)Immunoreactivity for calponin, along with alpha-smooth muscle actin and smoothmuscle myosin heavy chains, confirms the known neoplastic myoepithelial componentof adenoid cystic carcinomas and epithelial-myoepithelial carcinomas. The consistentlypositive staining pattern in adenoid cystic carcinomas may be diagnostically useful indiscriminating histologically similar but consistently negative polymorphous low-gradeadenocarcinomas (3). Alternate Names: anti-Basic calponin antibody, anti-CNN 1 antibody, anti-CNN1 antibody, anti-Calponin 1antibody, anti-Calponin 1 basic smooth muscle antibody, anti-Calponin H1 smoothmuscle antibody, anti-Calponin1 antibody, anti-Calponins basic antibody, anti-SMCCantibody, anti-Sm Calp antibody.

Spezifität: A synthetic peptide corresponding to residues near the C-term of human Calponin wasused as an immunogen. Species Reactivity: Reacts in Human, Mouse, and Rat. Not tested in other species. Localization: Cytoplasmic.

Anwendungen

Applikationshinweise: Suggested working dilutions: immunohistochemistry 1:100 to 250 ,Western Blot 1:20,Immunocytochemistry 1:100 to 250

Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. Preservative: 0.01% sodium azide.

Lagerung: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis on human bladder lysate using anti-Calponin RabMAb (ABIN189195), dilution 1:20,000

Immunohistochemical staining of paraffin-embedded human smooth muscle using anti-Calponin RabMAb

Immunofluorescent staining of Hela cells using anti Calponin-1 RabMAb

Publikationen

Publikationen: Strasser, Gimona, Moessler et al.: "Mammalian calponin. Identification and expression of genetic variants." in: FEBS letters, Vol. 330, Issue 1, pp. 13-8, 1993 (PubMed).

Jeyasuria, Wetzel, Bradley et al.: "Progesterone-regulated caspase 3 action in the mouse may play a role in uterine quiescence during pregnancy through fragmentation of uterine myocyte contractile proteins." in: Biology of reproduction, Vol. 80, Issue 5, pp. 928-34, 2009 (PubMed).