X-ray Repair, Complementing Defective, Repair in Chinese Hamster (XRCC5) Antikörper

Antigen: X-ray Repair, Complementing Defective, Repair in Chinese Hamster (XRCC5)

Synonyme: KU80, KUB2, Ku86, NFIV, KARP1, KARP-1, FLJ39089

Klonalität: Monoklonal (MEM-54)

Wirt: Maus

Reaktivität: Human

Applikation: Immunfluoreszenz (IF)

Produktnummer: ABIN188995

Produktbeschreibung

Weitere Bezeichnung: XRCC5 / Ku80 / Ku86

Immunogen: human whole T lymphocytes.

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: MEM-54

Beschreibung: Ku Antigen is a heterodimer (of 72 and 87 kDa polypeptides) which contributes togenomic integrity through its ability to bind DNA double-strand breaks and facilitaterepair by the nonhomologous end-joining pathway. A DNA double-strand break is amajor lesion that destroys the integrity of the DNA molecule. Such damage is introducedby ionizing radiation. Ku binds to free double-stranded DNA ends and is theDNA-binding component of the DNA-dependent protein kinase. Thus, the Ku protein isinvolved in DNA repair and in V(D)J recombination, and the Ku-DNA-dependent proteinkinase complex may have a role in those same processes. Ku70 and Ku80 share acommon topology and form a dyad-symmetrical molecule with a preformed ring thatencircles duplex DNA. The binding site can cradle 2 full turns of DNA while encirclingonly the central 3-4 base pairs. Ku makes no contacts with DNA bases and few with thesugar-phosphate backbone, but it fits sterically to major and minor groove contours soas to position the DNA helix in a defined path through the protein ring. These featuresare well designed to structurally support broken DNA ends and to bring the DNA helixinto phase across the junction during end processing and ligation. Mouse cells deficientfor Ku80 display a marked increase in chromosomal aberrations, including breakage,translocations, and aneuploidy. Despite the observed chromosome instabilities, Ku80 -/-mice have only a slightly earlier onset of cancer. Loss of p53 synergizes with Ku80 topromote tumorigenesis such that all Ku80 -/- and p53 -/- mice succumb to disseminatedpro-B-cell lymphoma before 3 months of age. The p70/p80 complex binds to the ends ofdouble-stranded DNA in a cell cycle-dependent manner, being associated withchromosomes of interphase cells, followed by complete dissociation from thecondensing chromosomes in early prophase. Some patients with systemic lupuserythematosus produce very large amounts of autoantibodies to p70 and p80. Theautoantibody has been found in patients with autoimmune thyroid disease (Gravesdisease) as well as in those with lupus.

Spezifität: The antibody MEM-54 reacts with Ku80, a 80 kDa subunit of Ku autoantigen(heterodimer of 72 and 87 kDa polypeptides). Ku autoantigen is involved in DNA repairand in V(D)J recombination through its ability to bind DNA double-strand breaks. Species Reactivity: Cross reacts with Human.

Anwendungen

Applikationshinweise: Suggested working dilutions: immunofluorescence 2 ug/ml

Konzentration: .1 mg/ml

Buffer: PBS containing 0.2% (w/v) high-grade protease free BSA as a stabilizingagent. Preservative: 15 mM sodium azide.

Lagerung: Store in the dark at 6C. Do not freeze. Avoid prolonged exposure to light.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, theconditions

Beschränkungen: Nur für Forschungszwecke einsetzbar