Aktivieren Sie Javascript in Ihrem Browser, um unsere Webseite optimal zu nutzen.

H2AFX Antikörper (C-Term)

Name: H2AFX Antikörper (C-Term)
SKU: ABIN1882250
Price: 445.5 EUR
Availability: InStock

H2AFX Reaktivität: Human WB, IHC (p) Wirt: Maus Monoclonal 938CT5-1-1 unconjugated

Western blot analysis of lysates from 293, CEM, HepG2, Jurkat, Hela, Raji cell line(from left to right).

Immunohistochemical analysis of paraffin-embedded H.

IHC (p)

Immunohistochemical analysis of paraffin-embedded Human Thymus section using Pink1 am2199b.

IHC

IHC (p)

4 Abbildungen

(3 Referenzen)

Produktnummer ABIN1882250

Datenblatt (PDF)

Target Alle H2AFX Antikörper anzeigen

H2AFX (H2A Histone Family, Member X (H2AFX))
Bindungsspezifität
52

26

18

11

6

5

5

5

4

4

4

4

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 115-143, C-Term
Reaktivität
191

93

60

26

26

20

17

12

9

3

2

2

2

2

1
Human
Wirt
163

28

2
Maus
Passende Sekundärantikörper
Klonalität
128

65
Monoklonal
Konjugat
123

8

7

7

7

6

5

4

4

4

4

3

2

2

2

2

2

1
Dieser H2AFX Antikörper ist unkonjugiert
Applikation
147

53

51

43

36

27

27

25

16

12

8

4

4

4

4

2

2

2

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung

This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen

This H2AFX antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 115-143 amino acids from the C-terminal region of human H2AFX.

Klon

938CT5-1-1

Isotyp

IgG1

Top Product

Discover our top product H2AFX Primärantikörper
anti-H2A Histone Family, Member X (H2AFX) antibody
H2AFX Reaktivität: Human, Maus, Ratte WB, IHC, IF Wirt: Kaninchen Polyclonal unconjugated

anti-H2A Histone Family, Member X (H2AFX) antibody
H2AFX Reaktivität: Human WB, IHC, ELISA, IF, ChIP Wirt: Kaninchen Polyclonal unconjugated

anti-H2A Histone Family, Member X (H2AFX) (pSer139) antibody
H2AFX Reaktivität: Human, Maus WB, BI Wirt: Maus Monoclonal N1-431 unconjugated

anti-H2A Histone Family, Member X (H2AFX) (pSer139) antibody
H2AFX Reaktivität: Human WB, ELISA, IF, ChIP Wirt: Kaninchen Polyclonal unconjugated

anti-H2A Histone Family, Member X (H2AFX) (pSer140) antibody
H2AFX Reaktivität: Human WB, ELISA, IHC (p), FACS, ICC, IF (p), IF (cc), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-H2A Histone Family, Member X (H2AFX) (ubLys119) antibody
H2AFX Reaktivität: Human, Maus, Ratte WB, ELISA, IHC (p), ICC, IF (p), IF (cc), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

anti-H2A Histone Family, Member X (H2AFX) (pSer139) antibody
H2AFX Reaktivität: Human WB, ELISA, IF Wirt: Kaninchen Polyclonal unconjugated

anti-H2A Histone Family, Member X (H2AFX) (AA 1-30), (N-Term) antibody
H2AFX Reaktivität: Human WB, IHC (p) Wirt: Maus Monoclonal 980CT1-5-5 unconjugated

anti-H2A Histone Family, Member X (H2AFX) antibody
H2AFX Reaktivität: Human WB, IHC (p) Wirt: Maus Monoclonal 8C5 unconjugated

anti-H2A Histone Family, Member X (H2AFX) (pSer139) antibody
H2AFX Reaktivität: Human WB, IF, ICC Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

WB: 1:1000. IHC: 1:50. IHC-P: 1:25. IHC-P: 1:25

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Haltbarkeit

6 months
Stiff, ODriscoll, Rief, Iwabuchi, Löbrich, Jeggo: "ATM and DNA-PK function redundantly to phosphorylate H2AX after exposure to ionizing radiation." in: Cancer research, Vol. 64, Issue 7, pp. 2390-6, (2004) (PubMed).

Lukas, Melander, Stucki, Falck, Bekker-Jensen, Goldberg, Lerenthal, Jackson, Bartek, Lukas: "Mdc1 couples DNA double-strand break recognition by Nbs1 with its H2AX-dependent chromatin retention." in: The EMBO journal, Vol. 23, Issue 13, pp. 2674-83, (2004) (PubMed).

Park, Chan, Park, Oettinger, Kwon: "DNA-PK is activated by nucleosomes and phosphorylates H2AX within the nucleosomes in an acetylation-dependent manner." in: Nucleic acids research, Vol. 31, Issue 23, pp. 6819-27, (2003) (PubMed).
Target

H2AFX (H2A Histone Family, Member X (H2AFX))

Andere Bezeichnung

H2AFX (H2AFX Produkte)

Hintergrund

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Molekulargewicht

15145

NCBI Accession

NP_002096

UniProt

P16104

Pathways

Telomere Maintenance, DNA Reparatur, Positive Regulation of Response to DNA Damage Stimulus