Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) Antikörper

Details zu Produkt Nr. ABIN1882239, Anbieter: Anmelden zum Anzeigen
Antigen
  • erk1/2
  • mitogen-activated protein kinase
  • erk1/2
Reaktivität
Human, Maus
421
320
305
57
23
15
9
8
8
7
7
6
5
5
3
3
2
2
2
1
1
1
1
Wirt
Maus
350
77
7
Klonalität (Klon)
Monoklonal ()
Konjugat
Unkonjugiert
12
11
11
10
7
7
7
7
6
6
6
6
6
6
2
2
2
2
1
1
1
1
1
1
1
Applikation
Western Blotting (WB)
305
114
83
78
60
56
46
42
16
10
6
4
4
4
2
2
2
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Purified His-tagged Erk1/2 protein was used to produced this monoclonal antibody.
Klon 784CT7-6-3
Isotyp IgG2a
Reinigung This antibody is purified through a protein G column, followed by dialysis against PBS.
Andere Bezeichnung Erk1/2 (MAPK1/3 Antibody Abstract)
Hintergrund Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The the MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.

Synonyms: MAPK3,ERK1, PRKM3, Mitogen-activated protein kinase 3, Mitogen-activated protein kinase 3, ERT2, Mitogen-activated protein kinase 3, Extracellular signal-regulated kinase 1, Mitogen-activated protein kinase 3, Insulin-stimulated MAP2 kinase, Mitogen-activated protein kinase 3, MAP kinase isoform p44, Mitogen-activated protein kinase 3, Microtubule-associated protein 2 kinase, Mitogen-activated protein kinase 3, p44-ERK1,Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2,MAPK1, ERK2,PRKM1, PRKM2
UniProt P28482, P27361
Forschungsgebiet Signaling, Inflammation
Applikationshinweise WB: 1:1000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.36 mg/mL
Buffer Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Bilder des Herstellers
Western Blotting (WB) image for anti-Mitogen-Activated Protein Kinase 1/3 (MAPK1/3) antibody (ABIN1882239) Erk1/2 Antibody (ABIN1882239) western blot analysis in 293,MCF-7,Jurkat,mouse NIH/3T3...
Produkt verwendet in: Guo, Meng, Bai, Wei, Shi, Davis, Mao: "Prostaglandin F2? induces expression of activating transcription factor 3 (ATF3) and activates MAPK signaling in the rat corpus luteum." in: Acta histochemica, Vol. 117, Issue 2, pp. 211-8, 2015 (PubMed).

Zhao, Ye, Wu, Liu, Yang, Gao, Chen, Zhang, Yang, Li: "Sp1-CD147 positive feedback loop promotes the invasion ability of ovarian cancer." in: Oncology reports, Vol. 34, Issue 1, pp. 67-76, 2015 (PubMed).

Rönnstrand: "Signal transduction via the stem cell factor receptor/c-Kit." in: Cellular and molecular life sciences : CMLS, Vol. 61, Issue 19-20, pp. 2535-48, 2004 (PubMed).

Sano, Liu, Lane, Piletz, Lienhard: "Insulin receptor substrate 4 associates with the protein IRAS." in: The Journal of biological chemistry, Vol. 277, Issue 22, pp. 19439-47, 2002 (PubMed).

Allgemeine Veröffentlichungen Charest, Mordret, Harder, Jirik, Pelech: "Molecular cloning, expression, and characterization of the human mitogen-activated protein kinase p44erk1." in: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4679-90, 1993 (PubMed).

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