MBD1 Antikörper (C-Term)

Produktnummer ABIN1882100

Produktdetails anti-MBD1 Antikörper

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Bindungsspezifität: AA 376-405, C-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MBD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Aufreinigung: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This MBD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 376-405 amino acids from the C-terminal region of human MBD1.

Klon: RB2371

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: WB: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Referenzen für anti-MBD1 Antikörper (ABIN1882100)

Auriol, Billard, Magdinier, Dante: "Specific binding of the methyl binding domain protein 2 at the BRCA1-NBR2 locus." in: Nucleic acids research, Vol. 33, Issue 13, pp. 4243-54, (2005) (PubMed).

Details zu MBD1

Target: MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))

Andere Bezeichnung: MBD1 (MBD1 Produkte)

Synonyme: CXXC3 antikoerper, PCM1 antikoerper, RFT antikoerper, Cxxc3 antikoerper, CCDC11 antikoerper, methyl-CpG binding domain protein 1 antikoerper, MBD1 antikoerper, Mbd1 antikoerper

Hintergrund: DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD) (1). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA (2). MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases (3). In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract (4).

Molekulargewicht: 66607

NCBI Accession: NP_001191065, NP_001191066, NP_001191067, NP_001191068, NP_001191069, NP_001191070, NP_001191071, NP_001191072, NP_001191080, NP_002375, NP_056669, NP_056670, NP_056671, NP_056723

UniProt: Q9UIS9