ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 1 (ABCC1) Antikörper

Antigen: ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 1 (ABCC1)

Synonyme: MRP, ABCC, GS-X, MRP1, ABC29, DKFZp781G125, DKFZp686N04233

Klonalität: Monoklonal (MRPm6)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Durchflusszytometrie (FACS), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN187339

Produktbeschreibung

Weitere Bezeichnung: ABCC1 / MRP1

Immunogen: Bacterial fusion protein of MRP containing a segment of 170 amino acids in the carboxy-terminal end and part of the carboxy-proximal nucleotide binding domain of the protein.Mouse myeloma (SP2/0) x immunized mouse lymph node cells (Balb/C).

Format: Supernatant

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: MRPm6

Spezifität: Reacts with an internal epitope of Multidrug Resistance Associated Protein 1 (MRP1, ABCC1), a 180-195 kDa transmembrane transporter protein which is overexpressed in various human non-P-glycoprotein MDR tumor cell lines. Does not crossreact with human MDR1 or MDR3 gene products. Species Reactivity: Human. Others not tested.

Anwendungen

Applikationshinweise: Immunocytochemistry: (Acetone fixed cell preparations) - Use at a 1:20-1:50 dilution. Immunohistochemistry: (Acetone fixed frozen sections) - Use at a 1:20-1:50 dilution. (Formalin-fixed, paraffin-embedded tissues)- Use at a 1:20 dilution. For paraffin-embedded sections a 0.01 M citrate pretreatment, 10 min at 100 °C, improves the performance of MRPm6. Flow cytometry: After fixing cells in 10% (v/v) Lysing solution, use MRPm6 at a 1:20-1:50 dilution followed by FITC-conjugated anti-mouse IgG secondary antibody. Western blotting: Use at a 1:20-1:50 dilution, followed by incubation with anti-mouse-HRP. The optimal dilution for a specific application should be determined by the researcher.

Buffer: 1 mL (>200 tests) or 0.5 mL (>100 tests) containing ~250 µg IgG/mL in serum-free tissue culture supernatant with protein stabilizer and 0.1% sodium azide. The antibody solution has been filtered through a 0.22 micron filter. Culture medium: RPMI-1640, su

Lagerung: Store at 4 °C for short term. Aliquot and store at -20 °C for long term. Avoid repeated freeze/thaw cycles.

Forschungsgebiet: Krebs

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 1 (ABCC1) antibody

Publikationen

Publikationen: Harris, Jeffery: "Sulforaphane and erucin increase MRP1 and MRP2 in human carcinoma cell lines." in: The Journal of nutritional biochemistry, Vol. 19, Issue 4, pp. 246-54, 2008 (PubMed).

Liao, Robey, Guirouilh-Barbat et al.: "Reduced expression of DNA topoisomerase I in SF295 human glioblastoma cells selected for resistance to homocamptothecin and diflomotecan." in: Molecular pharmacology, Vol. 73, Issue 2, pp. 490-7, 2008 (PubMed).

Roberts, Black, Raman et al.: "Subcellular localization of transporters along the rat blood-brain barrier and blood-cerebral-spinal fluid barrier by in vivo biotinylation." in: Neuroscience, Vol. 155, Issue 2, pp. 423-38, 2008 (PubMed).

Robey, Obrzut, Shukla et al.: "Becatecarin (rebeccamycin analog, NSC 655649) is a transport substrate and induces expression of the ATP-binding cassette transporter, ABCG2, in lung carcinoma cells." in: Cancer chemotherapy and pharmacology, Vol. 64, Issue 3, pp. 575-83, 2009 (PubMed).