ABCC2 / CMOAT / MRP2 Antikörper

Antigen: ABCC2 / CMOAT / MRP2

Klonalität: Monoklonal (M2 II-12)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Durchflusszytometrie (FACS), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN187320

Produktbeschreibung

Immunogen: Bacterial fusion protein containg amino acids 860-950 of human cMOAT/MRP2.

Format: Supernatant

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: M2 II-12

Beschreibung: Human cMOAT/MRP2 is a 190-200 kD transmembrane protein known as the canalicular multi-organic anion transporter. It is absent in humans with the Dubin-Johnson syndrome, an autosomal recessive liver disorder characterized by chronic conjugated hyperbilirubinemia. cMOAT/MRP2 is closely related to the multidrug resistance-related protein MRP, and cMOAT/MRP2 is overexpressed in a subset of cisplatin-resistant cell lines.

Spezifität: Reacts with an internal epitope of human cMOAT/MRP2. Does not cross-react with human MDR1, MRP1, MRP3, and MRP5 gene products. Species Reactivity: Human, others not tested.

Anwendungen

Applikationshinweise: Western blotting: Use 1:20-50 dilution and anti-mouse HRP. Immunohistochemistry: Use 1:20 on acetone fixed frozen sections can be followed by incubation with rabbit anti-mouse IgG and a monoclonal mouse APAAP complex. Immunocytochemistry: Use 1:20-50 dilution on acetone fixed cytospin preparations. Flow cytometry: Optimal conditions still to be defined. The optimal dilution for a specific application should be determined by the researcher.

Buffer: 0.5 ml of serum free cell culture supernatant concentrated to 250 µg/ml. Contains 0.7% BSA and 0.1% sodium azide.

Lagerung: Stable for at least 2 months when stored at 2-8 °C, and 1 year at -20 °C. Avoid repeated freeze/thaws.

Beschränkungen: Nur für Forschungszwecke einsetzbar