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Antikörper
»
anti-Thymine Dimer HRP
Thymine Dimer Antikörper (HRP)
| Antigen |
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| Klonalität |
Monoklonal (KTM53) |
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Wirt
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Reaktivität
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Human, Affe
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Konjugat
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HRP
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Applikation
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| Produktnummer |
ABIN187268 |
| Menge |
10 µg |
| Preis |
610,00 € Zzgl. Versandkosten €20,00 und MWSt
|
| Lieferung nach |
|
| Verfügbarkeit |
Versandbereit innerhalb von 7 bis 10 Werktagen |
|
Immunogen
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UV-irradiated salmon sperm DNAMouse myeloma (P3/X63-Ag8) x immunized mouse (Balb/c) splenocytes.
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Isotyp
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IgG1
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Klon
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KTM53
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Beschreibung
|
Horseradish peroxidase labeled monoclonal antibody that recognizes thymine dimers in DNA. Labeling ratio (antibody:horseradish peroxidase) = 1:2-1:4.
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Spezifität
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Reacts specifically with thymine dimers in double- or single-stranded DNA produced by UV irradiation. Does not react with (6-4) photo products.
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Applikationshinweise
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In Situ Hybridization: Use at a 1:40 to 1:80 dilution. The dilution factor depends on the application. Frozen sections of fixed tissue or formalin-fixed, paraffin-embedded sections are recommended. Southern, Northern, and Dot blot: For the detection of thymine dimers in UV-damaged DNA and thymine-dimerized DNA probes. (Reported to work, but no data is available) The optimal dilution for a specific application should be determined by the researcher.
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Buffer
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100 µL of 100 µg/mL HRP-labeled monoclonal antibody in PBS, purified by Protein A chromatography. Unlabeled antibody and peroxidase were removed by gel filtration.
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Lagerung
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Store at -20 °C. Aliquot to avoid freeze/thaw cycles.
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Forschungsgebiet
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DNS/RNS, Krebs
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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