anti-Thymine Dimer Antikörper (HRP)

Übersicht

Antigen:	Thymine Dimer
Klonalität:	Monoklonal (KTM53)
Applikation:	In situ hybridization (ISH)
Reaktivität:	Human, Monkey
Wirt:	Mouse
Konjugat:	HRP
Menge:	10 µg
Preis:	610,00 € (Zzgl. Versandkosten und MWSt)
Bestellnummer:	ABIN187268
Verfügbarkeit:	Versandbereit innerhalb von 7 bis 10 Werktagen

Antigen	Thymine Dimer
Immunogen	UV-irradiated salmon sperm DNAMouse myeloma (P3/X63-Ag8) x immunized mouse (Balb/c) splenocytes.
Reaktivität	Human, Monkey
Klonalität	Monoklonal
Isotyp	IgG1 »Passende Sekundärantikörper
Beschreibung	Horseradish peroxidase labeled monoclonal antibody that recognizes thymine dimers in DNA. Labeling ratio (antibody:horseradish peroxidase) = 1:2-1:4.
Klon	KTM53
Wirt	Mouse
Spezifität	Reacts specifically with thymine dimers in double- or single-stranded DNA produced by UV irradiation. Does not react with (6-4) photo products.

Applikation	In situ hybridization (ISH)
Applikationshinweise	In Situ Hybridization: Use at a 1:40 to 1:80 dilution. The dilution factor depends on the application. Frozen sections of fixed tissue or formalin-fixed, paraffin-embedded sections are recommended. Southern, Northern, and Dot blot: For the detection of thymine dimers in UV-damaged DNA and thymine-dimerized DNA probes. (Reported to work, but no data is available) The optimal dilution for a specific application should be determined by the researcher.
Konjugat	HRP
Buffer	100 µL of 100 µg/mL HRP-labeled monoclonal antibody in PBS, purified by Protein A chromatography. Unlabeled antibody and peroxidase were removed by gel filtration.
Lagerung	Store at -20 °C. Aliquot to avoid freeze/thaw cycles.
Forschungsgebiet	DNS/RNS, Krebs
Beschränkungen	Nur für Forschungszwecke einsetzbar