Perforin 1 / PRF1 (189-360) Antikörper

Antigen: Perforin 1 / PRF1 (189-360)

Klonalität: Monoklonal (KM585 (P1-8))

Wirt: Ratte

Reaktivität: Human, Maus

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Enzyme Immunoassay (EIA), Durchflusszytometrie (FACS), Immunfluoreszenz (IF), Western Blot (WB)

Produktnummer: ABIN187239

Produktbeschreibung

Immunogen: Recombinant mouse perforin fragment encompassing 172 amino acid residues (His189-Cys360) which contains the membrane binding domain and part of the EGF-type cysteine-rich domain.Mouse myeloma (P3U/I) x immunized SD rat spleen cells.

Format: Ascites

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: KM585 (P1-8)

Spezifität: Reacts with the common epitope of perforin existing in NK cells and CTL cells. Recognizes mouse perforin, cross-reacts with human perforin. Species Reactivity: Mouse and human. Others not tested.

Anwendungen

Applikationshinweise: ELISA. Western blot. Immunohistochemistry: Frozen tissue sections and cytospins. Use at a 1:50-1:200 dilution. It is recommended that treatment with H2O2 in water be substituted for H2O2 in methanol. Flow cytometry: Successful application of clone KM585 (P1-8) to flow cytometry has been reported by some users. The optimal dilution for a specific application should be determined by the researcher.

Buffer: 100 µL of monoclonal antibody in ascites fluid containing 0.1% sodium azide.

Lagerung: Store at 4 °C short term. For long term, store at -20 °C. Aliquot to avoid freeze/thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Araki, Fann, Wersto et al.: "Histone acetylation facilitates rapid and robust memory CD8 T cell response through differential expression of effector molecules (eomesodermin and its targets: perforin and granzyme B)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 12, pp. 8102-8, 2008 (PubMed).

Motegi, Kinoshita, Inatsu et al.: "IL-15-induced CD8+CD122+ T cells increase antibacterial and anti-tumor immune responses: implications for immune function in aged mice." in: Journal of leukocyte biology, Vol. 84, Issue 4, pp. 1047-56, 2008 (PubMed).

Urrea Moreno, Gil, Rodriguez-Sainz et al.: "Functional assessment of perforin C2 domain mutations illustrates the critical role for calcium-dependent lipid binding in perforin cytotoxic function." in: Blood, Vol. 113, Issue 2, pp. 338-46, 2009 (PubMed).

Saha, Bhattacharya-Chatterjee, Foon et al.: "Stimulatory effects of CpG oligodeoxynucleotide on dendritic cell-based immunotherapy of colon cancer in CEA/HLA-A2 transgenic mice." in: International journal of cancer. Journal international du cancer, Vol. 124, Issue 4, pp. 877-88, 2008 (PubMed).