ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1) Antikörper

Antigen: ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1)

Synonyme: CLCS, MDR1, P-GP, PGY1, ABC20, CD243, GP170, MGC163296, Abcb1, Mdr1a, ABCB1, ABCB4, CMDR1, xemdr, p-gp

Klonalität: Monoklonal (MRK16)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Durchflusszytometrie (FACS), Funktionale Studien (Func), Immunpräzipitation (IP)

Produktnummer: ABIN187222

Produktbeschreibung

Weitere Bezeichnung: CD243 / MDR1

Gen-ID: 5243

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: MRK16

Beschreibung: The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. [provided by RefSeq] Also known as: CLCS, MDR1, P-GP, PGY1, ABC20, CD243, GP170, MGC163296, ABCB1.

Anwendungen

Applikationshinweise: Potential Uses: cancer multidrug resistance studies, anti-cancer agent screening, AIDS multi-drug resistance studies, nervous system research, immunology research and transplant rejection studies, endocrine/steroid research, endothelium cell and brain P-glycoprotein research. Flow cytometry: For 5 x 105 pelleted cells use 50 µL of 10-100 µg/mL. Immunoprecipitation: Generally use between 25 µg/mL and 50 µg/mL. Immunohistochemistry: Generally use between 0.1 µg/mL and 10 µg/mL. Appropriate dilution must be decided upon according to staining method, conditions, etc. Frozen tissue staining can be done provided that mild fixation treatment is used, such as 4% paraformaldehyde. Acetone, paraffin, methyl alcohol, and ethyl alcohol should not be used as a fixative. Iodine labeling can also be performed on MRK16. Western blot: Does not react. Apparently, the epitope recognized by this antibody is lost in the presence of protein denaturing agents such as SDS. The optimal dilution for a specific application should be determined by the researcher.

Buffer: 300 µL of 500 µg/mL monoclonal antibody in PBS, with protein stabilizer, 0.1% sodium azide. Purified by Protein A affinity chromatography. Page 1 of 2

Lagerung: Store at -20 °C. Avoid repeated freeze/thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: de Figueiredo-Pontes, Pintao, Oliveira et al.: "Determination of P-glycoprotein, MDR-related protein 1, breast cancer resistance protein, and lung-resistance protein expression in leukemic stem cells of acute myeloid leukemia." in: Cytometry. Part B, Clinical cytometry, Vol. 74, Issue 3, pp. 163-8, 2008 (PubMed).

Valera, de Freitas Cortez, de Paula Queiroz et al.: "Pediatric glioblastoma cell line shows different patterns of expression of transmembrane ABC transporters after in vitro exposure to vinblastine." in: Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery, Vol. 25, Issue 1, pp. 39-45, 2008 (PubMed).