DIO3 Antikörper (AA 11-277)
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- Target Alle DIO3 Antikörper anzeigen
- DIO3 (Deiodinase, Iodothyronine, Type III (DIO3))
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Bindungsspezifität
- AA 11-277
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Reaktivität
- Maus
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser DIO3 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Sequenz
- MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDE ALKDAQTGST SGSGHHHHHH SAGLVPRGST AIGMKETAAA KFERQHMDSP DLGTLEVLFQ GPLGSEF- RLCAQTASCL VLFPRFLGTA FMLWLLDFLC IRKHFLRRRH PDHPEPEVEL NSEGEEMPPD DPPICVSDDN RLCTLASLKA VWHGQKLDFF KQAHEGGPAP NSEVVRPDGF QSQRILDYAQ GTRPLVLNFG SCTUPPFMAR MSAFQRLVTK YQRDVDFLII YIEEAHPSDG WVTTDSPYVI PQHRSLEDRV SAARVLQQGA PGCALVLDTM ANSSSSAYGA YFERLYVIQS GTIMYQGGRG PDGYQVSELR TWLERYDEQL HGTRPHR
- Spezifität
- It has been selected for its ability to recognize DIO3 in immunohistochemical staining and Western blotting.
- Aufreinigung
- Affinity Chromatography
- Immunogen
- DIO3 (AA 11-277)
- Isotyp
- IgG
- Top Product
- Discover our top product DIO3 Primärantikörper
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- Applikationshinweise
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Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user. - Kommentare
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Content: The quality control contains recombinant DIO3 (Arg11~Arg277) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % . - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handhabung
- Avoid repeated freeze/thaw cycles
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
- Haltbarkeit
- 12 months
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- Target
- DIO3 (Deiodinase, Iodothyronine, Type III (DIO3))
- Abstract
- DIO3 Produkte
- Synonyme
- 5DIII antikoerper, DIOIII antikoerper, txdi3 antikoerper, dioiii antikoerper, XL-15 antikoerper, itdi3 antikoerper, D3 antikoerper, TXDI3 antikoerper, iodothyronine deiodinase 3 antikoerper, deiodinase, iodothyronine, type 3 antikoerper, deiodinase, iodothyronine, type III antikoerper, deiodinase, iodothyronine type III antikoerper, deiodinase, iodothyronine, type 3 L homeolog antikoerper, DIO3 antikoerper, dio3 antikoerper, Dio3 antikoerper, dio3.L antikoerper
- Pathways
- Hormone Activity
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