CA12 (AA 161-318) Antikörper

Details zu Produkt Nr. ABIN1858202, Anbieter: Anmelden zum Anzeigen
Antigen
  • CAXII
  • HsT18816
  • CAR12
  • 2310047E01Rik
  • AI314958
  • Ca12
  • carbonic anhydrase 12
  • CA12
  • Car12
Epitop
AA 161-318
12
11
10
7
5
5
5
4
4
4
3
2
2
1
1
1
1
1
Reaktivität
Maus
120
35
29
1
1
Wirt
Kaninchen
77
44
13
Klonalität
Polyklonal
Konjugat
Unkonjugiert
13
12
7
3
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
82
64
19
17
13
8
7
7
5
5
3
2
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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'Independent Validation' Siegel
Antigen CA12
Chargennummer A20170109527
Validierte Anwendung Immunohistochemistry
Positivkontrolle mouse kidney, mouse ciliary body
Negativkontrolle secondary antibody only
Primärantikörper ABIN3204699
Sekundärantikörper goat anti-rabbit IgG Alexa Fluor 488 antibody (Life Technology, A-11006, lot 1348202)
Protokoll
  • Cryo-section:
    • Wash mouse eye and kidney tissue in PBS.
    • Cryo-protection. Suspend tissue in Optimum Cutting Temperature (OCT) embedding media (Thermo Scientific, Lot 03801137).
    • Gently freeze suspended tissue in liquid nitrogen for 5min.
    • Sections performed on a cryostat (LEICA, CM3050S) set to 12µm.
    • Fix sections in 4% paraformaldehyde (PFA) for 5min at room temperature.
    • Rinse sections in PBS 3 times for 5min.
    • Block sections with 5% normal goat serum for 1h at RT.
  • Immunostaining:
    • Incubate sections with primary rabbit-anti CA12 antibody (antibodies-online, ABIN3204699, lot A20170109527) diluted 1:100 in PBSA, overnight at 4°C. Include a negative control without primary antibody and a kidney positive control.
    • Rinse sections with PBS 3 times for 5min. Keep negative control in a separate jar.
    • Incubate sections with secondary goat anti-rabbit IgG Alexa Fluor 488 antibody conjugate (Life Technology, A-11006, lot 1348202) diluted 1:500 in 1% Bovine Serum Albumin in PBS (PBSA) buffer for 2h at RT.
    • Counterstain with DAPI in the mounting medium step.
  • Wash and mount:
    • Rinse sections in PBS 3 times for 5min.
    • Mount sections in Vectorshield mounting medium with DAPI (Vector laboratories, H-1200, lot ZC0814).
  • Microscopy:
    • Image acquisition on an EVOS FL Color fluorescence and transmitted light microscope (Life technologies, Cat no. AMEFC4300) at 20x magnification.
Validierungsbilder
Immunohistochemistry validation image for anti-CA12 (AA 161-318) antibody (ABIN3204699) Immunostaining of murine kidney tissue (A.) and ciliary body sections (B.) with the C...
Immunogen CA12 (Ala161-Ile318)
Isotyp IgG
Spezifität The antibody is a rabbit polyclonal antibody raised against CA12. It has been selected for its ability to recognize CA12 in immunohistochemical staining and western blotting.
Reinigung Antigen-specific affinity chromatography
Andere Bezeichnung Carbonic Anhydrase XII (CA12)
Hintergrund Alternative Names: CA-XII, CAXII, Carbonate dehydratase XII, Tumor antigen HOM-RCC-3.1.3
Applikationshinweise
  • Western blotting: 0.5-2 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Optimal working dilutions must be determined by end user.
Kommentare

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 200 μg/mL
Buffer 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung 4 °C
Informationen zur Lagerung Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Haltbarkeit 12 months
Bilder des Herstellers
Western Blotting (WB) image for anti-CA12 (AA 161-318) antibody (ABIN1858202) anti-CA12 (AA 161-318) antibody
Western Blotting (WB) image for anti-CA12 (AA 161-318) antibody (ABIN1858202) Figure. Western Blot; Lane1: Human Blood Cells; Lane2: Rat Blood Cells; Lane3: Mouse ...
 image for anti-CA12 (AA 161-318) antibody (ABIN1858202) Figure.DAB staining on IHC-P. Samples: Mouse Tissue
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