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EN2 Antikörper (C-Term)

Verified EN2 Reaktivität: Human, Maus WB, ELISA, IF Wirt: Ziege Polyclonal unconjugated
Produktnummer ABIN185576
  • Target Alle EN2 Antikörper anzeigen
    EN2 (Engrailed Homeobox 2 (EN2))
    Bindungsspezifität
    • 18
    • 15
    • 12
    • 5
    • 1
    C-Term
    Reaktivität
    • 56
    • 33
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Maus
    Wirt
    • 54
    • 2
    Ziege
    Klonalität
    • 54
    • 2
    Polyklonal
    Konjugat
    • 23
    • 6
    • 5
    • 5
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser EN2 Antikörper ist unkonjugiert
    Applikation
    • 50
    • 30
    • 13
    • 13
    • 9
    • 8
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF)
    Verwendungszweck
    Engrailed-2
    Sequenz
    NHSTTAKEGK SDSE
    Kreuzreaktivität
    Hund, Human, Maus, Ratte
    Aufreinigung
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Güteklasse
    Verified
    Immunogen
    Peptide with sequence C-NHSTTAKEGKSDSE, from the C Terminus of the protein sequence according to NP_001418.2.
    Isotyp
    IgG
    Top Product
    Discover our top product EN2 Primärantikörper
  • Applikationshinweise

    Western Blot: Approx 35 kDa band observed in Mouse Brain lysates (calculated MW of f 33.8 kDa according to Mouse NP_034264.1). Recommended concentration: 0.1-1 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:4000.

    Kommentare

    Immunofluorescence: Strong expression of the protein seen in the nuclei of HEK293 and U2OS cells. Recommended concentration: 10μg/ml.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Minimize freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    EN2 (Engrailed Homeobox 2 (EN2))
    Andere Bezeichnung
    EN2 (EN2 Produkte)
    Synonyme
    EN2 antikoerper, en antikoerper, en2 antikoerper, BB131122 antikoerper, En-2 antikoerper, RGD1561842 antikoerper, Gg-En-2 antikoerper, engrailed homeobox 2 antikoerper, engrailed homeobox antikoerper, engrailed 2 antikoerper, EN2 antikoerper, en antikoerper, En2 antikoerper
    Hintergrund
    Engrailed-2, engrailed homolog 2 , EN2, AUTS1
    Gen-ID
    2020
    NCBI Accession
    NP_001418
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