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PEPCK Antikörper (Internal Region)

Verified PEPCK Reaktivität: Human, Maus WB, ELISA, IF, FACS Wirt: Ziege Polyclonal unconjugated
Produktnummer ABIN185483
  • Target Alle PEPCK Antikörper anzeigen
    PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))
    Bindungsspezifität
    • 15
    • 9
    • 8
    • 6
    • 5
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reaktivität
    • 52
    • 25
    • 11
    • 1
    Human, Maus
    Wirt
    • 60
    • 7
    Ziege
    Klonalität
    • 52
    • 15
    Polyklonal
    Konjugat
    • 26
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser PEPCK Antikörper ist unkonjugiert
    Applikation
    • 50
    • 26
    • 18
    • 14
    • 14
    • 10
    • 6
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Verwendungszweck
    PCK2 / PEPCK-M
    Sequenz
    KPWKPGDKEP CAH
    Spezifität
    No cross-reactivity with PCK1.
    Kreuzreaktivität
    Rind (Kuh), Hund, Human, Maus, Schwein, Ratte
    Aufreinigung
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Güteklasse
    Verified
    Immunogen
    Peptide with sequence KPWKPGDKEPCAH, from the internal region of the protein sequence according to NP_004554.2.
    Isotyp
    IgG
  • Applikationshinweise

    Western Blot: Approx 70 kDa band observed in lysates of cell lines A431, HEK293 HepG2 and NIH3T3 (calculated MW of 70.7 kDa according to Human NP_004554.3 and 70.5 kDa according to Mouse NP_083270.2). Recommended concentration: 1-3 μg/mL. Primary incubatio

    Peptide ELISA: antibody detection limit dilution 1:4000.

    Kommentare

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.<

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Minimize freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Méndez-Lucas, Duarte, Sunny, Satapati, He, Fu, Bermúdez, Burgess, Perales: "PEPCK-M expression in mouse liver potentiates, not replaces, PEPCK-C mediated gluconeogenesis." in: Journal of hepatology, Vol. 59, Issue 1, pp. 105-13, (2013) (PubMed).

  • Target
    PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))
    Andere Bezeichnung
    PCK2 (PEPCK Produkte)
    Synonyme
    PEPCK antikoerper, PEPCK-M antikoerper, PEPCK2 antikoerper, 1810010O14Rik antikoerper, 9130022B02Rik antikoerper, pck antikoerper, zgc:77867 antikoerper, id:ibd5157 antikoerper, pck2 antikoerper, pck2.S antikoerper, phosphoenolpyruvate carboxykinase 2, mitochondrial antikoerper, phosphoenolpyruvate carboxykinase 2 (mitochondrial) antikoerper, phosphoenolpyruvate carboxykinase 2 (mitochondrial) L homeolog antikoerper, PCK2 antikoerper, Pck2 antikoerper, pck2 antikoerper, pck2.L antikoerper
    Hintergrund
    PCK2, PEPCK-M, phosphoenolpyruvate carboxykinase 2 (mitochondrial), HGNC:8725, PEPCK, PEPCK2 , PEP carboxykinase, mitochondrial phosphoenolpyruvate carboxykinase 2, phosphoenolpyruvate carboxylase, phosphopyruvate carboxylase
    Gen-ID
    5106
    NCBI Accession
    NP_004554
    Pathways
    Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
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