VPS35 Antikörper (C-Term)

Produktnummer ABIN185182

Produktdetails anti-VPS35 Antikörper

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser VPS35 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Verwendungszweck: VPS35 / MEM3

Sequenz: SPESEGPIYE GLIL

Spezifität: Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.

Kreuzreaktivität: Rind (Kuh), Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence C-SPESEGPIYEGLIL, from the C Terminus of the protein sequence according to NP_060676.2.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Prostate. Recommended concentration: 8 μg/mL.

Western Blot: Approx 90 kDa band observed in Human (Cerebellum), Mouse and Rat Brain lysates and in lysates of cell line HepG2, and approx. 85 kDa observed in lysates of cell line HEK293 (calculated MW of 91.7 kDa according to Human NP_060676.2, Mouse NP_0

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/vesicles of HEK293 and U2OS cells. Recommended concentration: 10μg/ml.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Referenzen für anti-VPS35 Antikörper (ABIN185182)

Klinger, Højland, Jain, Kjolby, Madsen, Svendsen, Olivecrona, Bonifacino, Nielsen: "Polarized trafficking of the sorting receptor SorLA in neurons and MDCK cells." in: The FEBS journal, Vol. 283, Issue 13, pp. 2476-93, (2017) (PubMed).

Lee, Uchida, Emoto, Umeda, Kuge, Taguchi, Arai: "Impaired retrograde membrane traffic through endosomes in a mutant CHO cell defective in phosphatidylserine synthesis." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 17, Issue 8, pp. 728-36, (2012) (PubMed).

Details zu VPS35

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Andere Bezeichnung: VPS35 (VPS35 Produkte)

Synonyme: MEM3 antikoerper, PARK17 antikoerper, vps35 antikoerper, MGC81959 antikoerper, bZ34G2.3 antikoerper, si:rp71-34g2.3 antikoerper, wu:fa66e03 antikoerper, wu:fb95c08 antikoerper, zgc:136268 antikoerper, 23.m05999 antikoerper, Mem3 antikoerper, VPS35 antikoerper, DKFZp459E1510 antikoerper, CG5625 antikoerper, DVps35 antikoerper, Dmel\\CG5625 antikoerper, Dvps35 antikoerper, dvps35 antikoerper, AI647796 antikoerper, mem3 antikoerper, VPS35, retromer complex component antikoerper, VPS35 retromer complex component L homeolog antikoerper, VPS35 retromer complex component antikoerper, vacuolar protein sorting 35 homolog (S. cerevisiae) antikoerper, vacuolar protein sorting-associated protein 35 antikoerper, vacuolar protein sorting 35 antikoerper, Vacuolar protein sorting 35 antikoerper, hypothetical protein antikoerper, VPS35 retromer complex component S homeolog antikoerper, retromer complex subunit Vps35 antikoerper, VPS35 antikoerper, vps35.L antikoerper, vps35 antikoerper, Tc00.1047053511367.170 antikoerper, Tb10.70.5460 antikoerper, BBOV_IV009740 antikoerper, CC1G_12185 antikoerper, PTRG_08682 antikoerper, CMU_017400 antikoerper, PHATR_43830 antikoerper, MCYG_00751 antikoerper, MGYG_01212 antikoerper, EHI_086580 antikoerper, EHI_002990 antikoerper, EHI_041950 antikoerper, EHI_096520 antikoerper, Bm1_44045 antikoerper, LOC100285267 antikoerper, Vps35 antikoerper, PGTG_10018 antikoerper, Tsp_09825 antikoerper, vps35.S antikoerper

Hintergrund: VPS35, MEM3, FLJ10752, FLJ13588, FLJ20388, DKFZp434E1211, DKFZp434P1672, vacuolar protein sorting 35 (yeast), maternal-embryonic 3, vacuolar protein sorting 35 homolog (S. cerevisiae), vacuolar protein sorting 35

Gen-ID: 55737, 65114

NCBI Accession: NP_060676