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Melanophilin Antikörper (C-Term)

Verified MLPH Reaktivität: Maus WB, ELISA, IF, ICC, Pull-Down Wirt: Ziege Polyclonal unconjugated
Produktnummer ABIN184753
  • Target Alle Melanophilin (MLPH) Antikörper anzeigen
    Melanophilin (MLPH)
    Bindungsspezifität
    • 10
    • 8
    • 7
    • 6
    • 2
    • 1
    • 1
    • 1
    C-Term
    Reaktivität
    • 36
    • 8
    • 3
    • 1
    Maus
    Wirt
    • 36
    • 4
    Ziege
    Klonalität
    • 38
    • 2
    Polyklonal
    Konjugat
    • 18
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Melanophilin Antikörper ist unkonjugiert
    Applikation
    • 30
    • 21
    • 20
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Pull-Down Assay (Pull-Down)
    Verwendungszweck
    Melanophilin (mouse)
    Sequenz
    ARHIFAKPVM AQQP
    Kreuzreaktivität
    Maus, Ratte
    Aufreinigung
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Güteklasse
    Verified
    Immunogen
    Peptide with sequence C-ARHIFAKPVMAQQP, from the C Terminus of the protein sequence according to NP_443748.2.
    Isotyp
    IgG
    Top Product
    Discover our top product MLPH Primärantikörper
  • Applikationshinweise

    Western Blot: Approx 80 kDa band observed in Mouse Melanocyte extracts. Recommended for use at 0.5-2 μg/mL. Please note that this product may require overnight incubation of primary and high protein loads. This antibody has been successfully used in WB on

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Kommentare

    Immunofluorescence: Successfully used at 0.5ug/ml to stain mouse melanocytes. This antibody has been successfully used in IF on Mouse: Figueiredo AC et al., J Biol Chem. 2008 Aug 22,283(34):23209-16. PMID:18559336.
    GST-pull do

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Minimize freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Sanzà, Evans, Briggs, Cantero, Montoliu, Patel, Sviderskaya, Itzen, Figueiredo, Seabra, Hume: "Nucleotide exchange factor Rab3GEP requires DENN and non-DENN elements for activation and targeting of Rab27a." in: Journal of cell science, Vol. 132, Issue 9, (2020) (PubMed).

    Singh, Mizuno, Wasmeier, Wavre-Shapton, Recchi, Catz, Futter, Tolmachova, Hume, Seabra: "Distinct and opposing roles for Rab27a/Mlph/MyoVa and Rab27b/Munc13-4 in mast cell secretion." in: The FEBS journal, Vol. 280, Issue 3, pp. 892-903, (2013) (PubMed).

    Figueiredo, Wasmeier, Tarafder, Ramalho, Baron, Seabra: "Rab3GEP is the non-redundant guanine nucleotide exchange factor for Rab27a in melanocytes." in: The Journal of biological chemistry, Vol. 283, Issue 34, pp. 23209-16, (2008) (PubMed).

    Hume, Tarafder, Ramalho, Sviderskaya, Seabra: "A coiled-coil domain of melanophilin is essential for Myosin Va recruitment and melanosome transport in melanocytes." in: Molecular biology of the cell, Vol. 17, Issue 11, pp. 4720-35, (2006) (PubMed).

  • Target
    Melanophilin (MLPH)
    Andere Bezeichnung
    Mlph (MLPH Produkte)
    Synonyme
    MGC155106 antikoerper, LOC100222096 antikoerper, 2210418F23Rik antikoerper, 5031433I09Rik antikoerper, AW228792 antikoerper, D1Wsu84e antikoerper, Slac-2a antikoerper, l(1)-3Rk antikoerper, l1Rk3 antikoerper, ln antikoerper, SLAC2-A antikoerper, melanophilin antikoerper, MLPH antikoerper, mlph antikoerper, Mlph antikoerper
    Hintergrund
    2210418F23Rik, melanophilin, Mlph, ln, l1Rk3, Slac-2a, D1Wsu84e, l(1)-3Rk, 5031433I09Rik, leaden, AW228792, OTTMUSP00000025451
    Gen-ID
    171531, 316620
    NCBI Accession
    NP_443748
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