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ARPC2 Antikörper (C-Term)

Verified ARPC2 Reaktivität: Human WB, ELISA, IHC, IF Wirt: Ziege Polyclonal unconjugated
Produktnummer ABIN184748
  • Target Alle ARPC2 Antikörper anzeigen
    ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))
    Bindungsspezifität
    • 15
    • 14
    • 7
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reaktivität
    • 57
    • 13
    • 11
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Wirt
    • 53
    • 4
    Ziege
    Klonalität
    • 51
    • 6
    Polyklonal
    Konjugat
    • 22
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser ARPC2 Antikörper ist unkonjugiert
    Applikation
    • 45
    • 22
    • 13
    • 13
    • 6
    • 6
    • 6
    • 5
    • 4
    • 4
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
    Verwendungszweck
    ARPC2
    Sequenz
    EMKTITGKTF SSR
    Spezifität
    The reported variants NP_005722.1 and NP_690601.1 represent identical protein.
    Kreuzreaktivität
    Rind (Kuh), Hund, Human, Maus, Ratte
    Aufreinigung
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Güteklasse
    Verified
    Immunogen
    Peptide with sequence C-EMKTITGKTFSSR, from the C Terminus of the protein sequence according to NP_005722.1, NP_690601.1.
    Isotyp
    IgG
    Top Product
    Discover our top product ARPC2 Primärantikörper
  • Applikationshinweise

    Immunohistochemistry: In paraffin embedded Human Spleen shows cytoplasm staining in some splenocytes in and around a T-cell area. Recommended concentration, 3-5 μg/mL.

    Western Blot: Approx 34-35 kDa band observed in lysates of cell lines CACO-2, HeLa and Jurkat (calculated MW of 34.3 kDa according to NP_005722.1). Recommended concentration: 0.1-0.5 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Kommentare

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10μg/ml.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Minimize freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Sakata, Taniguchi, Yasuda, Adachi-Morishima, Hamazaki, Nakayama, Miki, Minato, Narumiya: "Impaired T lymphocyte trafficking in mice deficient in an actin-nucleating protein, mDia1." in: The Journal of experimental medicine, Vol. 204, Issue 9, pp. 2031-8, (2007) (PubMed).

  • Target
    ARPC2 (Actin Related Protein 2/3 Complex, Subunit 2, 34kDa (ARPC2))
    Andere Bezeichnung
    ARPC2 (ARPC2 Produkte)
    Synonyme
    arc34 antikoerper, p34-arc antikoerper, pnas-139 antikoerper, pro2446 antikoerper, Arpc2 antikoerper, Arc-p34 antikoerper, 2210023N03Rik antikoerper, 34kDa antikoerper, p34-Arc antikoerper, fk84a07 antikoerper, wu:fa22f07 antikoerper, wu:fk84a07 antikoerper, zgc:77769 antikoerper, ARC34 antikoerper, PNAS-139 antikoerper, actin related protein 2/3 complex subunit 2 antikoerper, actin related protein 2/3 complex, subunit 2, 34kDa antikoerper, actin related protein 2/3 complex, subunit 2 antikoerper, actin related protein 2/3 complex subunit 2 S homeolog antikoerper, ARPC2 antikoerper, arpc2 antikoerper, Arpc2 antikoerper, arpc2.S antikoerper
    Hintergrund
    ARPC2, actin related protein 2/3 complex, subunit 2, 34 kDa, ARC34, PRO2446, p34-Arc, PNAS-139, ARP2/3 protein compex subunit 34, actin related protein 2/3 complex subunit 2
    Gen-ID
    10109, 76709
    NCBI Accession
    NP_005722, NP_690601
    Pathways
    RTK Signalweg, Regulation of Actin Filament Polymerization
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