GRB2 Antikörper (C-Term)

Produktnummer ABIN184670

Produktdetails anti-GRB2 Antikörper

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GRB2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Verwendungszweck: GRB2

Sequenz: PRNYVTPVNR NV

Spezifität: This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1).

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence C-PRNYVTPVNRNV, from the C Terminus of the protein sequence according to NP_002077.1, NP_987102.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Lymph Node shows nuclear staining in some cells inside the germinal centre. Recommended concentration: 1-2 μg/mL.

Western Blot: Approx 26 kDa band observed in lysates of cell line MOLT4, of Human Thymus and of Human, Mouse, Rat, Pig Brain and Spleen lysates (calculated MW of 25.2 kDa according to Human NP_002077.1 and according to Rat NP_110473.2). Recommended for use

Peptide ELISA: antibody detection limit dilution 1:32000.

Kommentare:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu GRB2

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Andere Bezeichnung: GRB2 (GRB2 Produkte)

Synonyme: GRB2 antikoerper, grb2 antikoerper, AA408164 antikoerper, Ash antikoerper, ASH antikoerper, EGFRBP-GRB2 antikoerper, Grb3-3 antikoerper, MST084 antikoerper, MSTP084 antikoerper, NCKAP2 antikoerper, wu:fb18e12 antikoerper, wu:fb34e06 antikoerper, zgc:73077 antikoerper, growth factor receptor bound protein 2 antikoerper, growth factor receptor-bound protein 2 antikoerper, growth factor receptor-bound protein 2b antikoerper, GRB2 antikoerper, grb2 antikoerper, Grb2 antikoerper, grb2b antikoerper

Hintergrund: GRB2, growth factor receptor-bound protein 2, ASH, ASH protein, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, HT027, abundant SRC homology, epidermal growth factor receptor-binding protein GRB2, growth factor receptor-bound protein 3, NCKAP2, OTTHUMP00000166096,

Gen-ID: 2885, 14784, 81504

NCBI Accession: NP_002077, NP_987102

Pathways: RTK Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation