ITK Antikörper (C-Term)

Produktnummer ABIN184605

Produktdetails anti-ITK Antikörper

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ITK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Verwendungszweck: ITK

Sequenz: RLLRQLAEIA ESGL

Kreuzreaktivität: Human, Maus

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence C-RLLRQLAEIAESGL, from the C Terminus of the protein sequence according to NP_005537.3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 75 kDa band observed in nuclear ysates of cell line Jurkat (calculated MW of 71.8 kDa according to NP_005537.3). Recommended concentration: 1-3g/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:2000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and Jurkat cells. Recommended concentration: 10μg/ml.

Flow Cytometry: A customer reported weak staining of Jurkat cells at a concentrat

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu ITK

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Andere Bezeichnung: ITK (ITK Produkte)

Synonyme: Emt antikoerper, Tcsk antikoerper, Tsk antikoerper, EMT antikoerper, LPFS1 antikoerper, LYK antikoerper, PSCTK2 antikoerper, IL2 inducible T cell kinase antikoerper, IL2 inducible T-cell kinase antikoerper, IL2-inducible T-cell kinase antikoerper, Itk antikoerper, ITK antikoerper

Hintergrund: ITK, EMT, LYK, PSCTK2, IL2-inducible T-cell kinase, tyrosine-protein kinase LYK, homolog of mouse T-cell itk/tsk, tyrosine-protein kinase ITK/TSK, MGC126257, MGC126258

Gen-ID: 3702, 16428

NCBI Accession: NP_005537

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung