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STAT3 Antikörper (C-Term)

Verified STAT3 Reaktivität: Human, Maus IHC, ELISA, IF, FACS Wirt: Ziege Polyclonal unconjugated
Produktnummer ABIN184593
  • Target Alle STAT3 Antikörper anzeigen
    STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))
    Bindungsspezifität
    • 97
    • 59
    • 26
    • 8
    • 7
    • 7
    • 7
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reaktivität
    • 290
    • 150
    • 149
    • 38
    • 27
    • 25
    • 24
    • 19
    • 17
    • 6
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Human, Maus
    Wirt
    • 228
    • 72
    • 3
    • 2
    • 1
    • 1
    Ziege
    Klonalität
    • 195
    • 110
    Polyklonal
    Konjugat
    • 169
    • 23
    • 17
    • 11
    • 11
    • 10
    • 9
    • 7
    • 7
    • 7
    • 7
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Dieser STAT3 Antikörper ist unkonjugiert
    Applikation
    • 224
    • 109
    • 76
    • 59
    • 50
    • 44
    • 42
    • 41
    • 27
    • 24
    • 15
    • 10
    • 8
    • 6
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Verwendungszweck
    STAT3 (isoform 1 and 2)
    Sequenz
    DMELTSECAT SPM
    Spezifität
    This antibody is expected to recognise isoforms 1 and 2 (as represented by NP_644805.1 and NP_003141.2 respectively)
    Kreuzreaktivität
    Rind (Kuh), Human, Maus, Ratte
    Aufreinigung
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Güteklasse
    Verified
    Immunogen
    Peptide with sequence DMELTSECATSPM, from the C Terminus of the protein sequence according to NP_003141.2, NP_644805.1.
    Isotyp
    IgG
    Top Product
    Discover our top product STAT3 Primärantikörper
  • Applikationshinweise

    Immunohistochemistry: In paraffin embedded Mouse Thymus shows preferential staining of medula over cortex. Recommended concentration: 4-6 μg/mL.

    Western Blot: Preliminary testing showed a band at approx 80-85 kDa in A431 nuclear cell lysate after 2 μg/mL antibody staining (calculated MW of 80 kDa according to NP_003141.2, and NP_644805.1). Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:8000.

    Kommentare

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and A431 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Minimize freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    STAT3 (Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3))
    Andere Bezeichnung
    STAT3 (STAT3 Produkte)
    Synonyme
    1110034C02Rik antikoerper, AW109958 antikoerper, Aprf antikoerper, APRF antikoerper, HIES antikoerper, Xstat3 antikoerper, aprf antikoerper, hies antikoerper, stat3 antikoerper, wu:fc15d02 antikoerper, wu:fl59g06 antikoerper, z-Stat3 antikoerper, signal transducer and activator of transcription 3 antikoerper, signal transduction and activation of transcription 3 antikoerper, signal transducer and activator of transcription 3, gene 1 L homeolog antikoerper, signal transducer and activator of transcription 3 (acute-phase response factor) antikoerper, STAT3 antikoerper, stat3 antikoerper, Stat3 antikoerper, stat3.1.L antikoerper
    Hintergrund
    STAT3, signal transducer and activator of transcription 3 (acute-phase response factor), APRF, DNA-binding protein APRF, acute-phase response factor, FLJ20882, MGC16063, HIES
    Gen-ID
    6774, 20848, 25125
    NCBI Accession
    NP_003141, NP_644805
    Pathways
    JAK-STAT Signalweg, RTK Signalweg, Interferon-gamma Pathway, Neurotrophin Signalübertragung, Dopaminergic Neurogenesis, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Stem Cell Maintenance, Hepatitis C, Protein targeting to Nucleus, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor
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