»
Antikörper
»
anti-6xHistidine Epitope Tag Antikörper
6xHistidine Epitope Tag Antikörper
| Antigen |
|
| Klonalität |
Polyklonal |
|
Wirt
|
|
|
Applikation
|
|
| Produktnummer |
ABIN184478 |
| Menge |
0.2 mg (200 μg/ml) |
| Preis |
300,00 € Zzgl. Versandkosten €40,00 und MWSt
|
| Lieferung nach |
|
| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
|
Weitere Bezeichnung
|
6xHistidine Epitope Tag (HHHHHH)
|
|
Immunogen
|
raised against a peptide containing the 6x histidine tag
|
|
Beschreibung
|
The 6x histidine recombinant protein tag is made up of 6 histidine residues attached to either the amino or carboxy terminus of the protein being expressed. The recombinant protein can then be purified using immobilized metal ions, traditionally nickel, in a procedure termed immobilized-metal affinity chromatography. Further analysis of the protein can be achieved using an antibody specific to the 6x histidine in techniques such as western blotting and immunoprecipitation.
|
|
Applikationshinweise
|
His (H20) is recommended for use by western blotting and immunoprecipitation. The suggested western blotting starting dilution is at 1:400.
|
|
Konzentration
|
200 μg/ml
|
|
Reinheit
|
affinity purified rabbit polyclonal antibody
|
|
Buffer
|
Each vial contains 200 μg/ml of affinity purified rabbit IgG, His (H20) DB063, in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
|
|
Lagerung
|
Store this product at 4º C, do not freeze. The product is stable for one year from the date of shipment.
|
|
Beschränkungen
|
Nur für Forschungszwecke einsetzbar
|
Alternativen zu Antigen "6xHistidine Epitope Tag", Typ "Antikörper" finden
|
Wirte
|
Maus (31), Kaninchen (23), Ziege (10), Huhn (7)
|
|
Reaktivitäten
|
Alle Spezies (3), Human (2)
|
|
Applikationen
|
Western Blot (WB) (63), Enzyme Immunoassay (EIA) (39), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (20), Immunfluoreszenz (IF) (18), Immunpräzipitation (IP) (18), ELISA (6), Durchflusszytometrie (FACS) (4), ELISA (Fangantikörper) (1), ELISA (Detektionsantikörper) (1), Immunhistochemie (Paraffinschnitte) (IHC (p)) (1)
|
|
Konjugate
|
FITC (10), HRP (10), Biotin (5), Agarose Beads (2), PE (1)
|
Alternativen 
