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V5 Epitope Tag Antikörper

WB, ELISA Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1842466
  • Target Alle V5 Epitope Tag Produkte
    V5 Epitope Tag
    Reaktivität
    Bitte anfragen
    Wirt
    • 60
    • 41
    • 10
    • 7
    • 6
    • 2
    • 1
    Kaninchen
    Klonalität
    • 57
    • 57
    • 10
    Polyklonal
    Konjugat
    • 66
    • 7
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser V5 Epitope Tag Antikörper ist unkonjugiert
    Applikation
    • 101
    • 59
    • 44
    • 44
    • 31
    • 30
    • 13
    • 10
    • 8
    • 6
    • 6
    • 6
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Sequenz
    GKPIPNPLLG LDST
    Spezifität
    Rabbit Anti-V5-tag Polyclonal Antibody specifically recognizes V5 tagged fusion proteins.
    Produktmerkmale
    V5 Tag Antibody is a useful tool in detection of recombinant proteins containing the V5 epitope.
    Aufreinigung
    Immunoaffinity chromatography
    Immunogen
    V5 epitope tag peptide GKPIPNPLLGLDST conjugated to KLH
    Isotyp
    IgG
  • Applikationshinweise
    Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors,
    Including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

    ELISA: 0. 05-0. 2 µg/mL
    Western blot: 0. 1-1. 0 µg/mL
    Western blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit L00204, C is recommended. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane. Other applications: user-optimized
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final antibody concentration of 0.5 mg/mL.
    Konzentration
    0.5 mg/mL
    Buffer
    lyophilized with PBS, pH 7.4, containing 0.02 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    The antibody is stable in lyophilized form if stored at -20 °C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -20 °C or below.
  • Afrazi, Sodhi, Good, Jia, Siggers, Yazji, Ma, Neal, Prindle, Grant, Branca, Ozolek, Chang, Hackam: "Intracellular heat shock protein-70 negatively regulates TLR4 signaling in the newborn intestinal epithelium." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 9, pp. 4543-57, (2012) (PubMed).

  • Target
    V5 Epitope Tag
    Abstract
    V5 Epitope Tag Produkte
    Substanzklasse
    Tag
    Hintergrund
    The V5 epitope tag is derived from a small epitope which presents on the P and V proteins of the paramyxovirus of simian virus 5 (SV5).
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