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IL12A Antikörper (Biotin)

IL12A Reaktivität: Maus WB, EIA Wirt: Ziege Polyclonal Biotin
Produktnummer ABIN181757
  • Target Alle IL12A Antikörper anzeigen
    IL12A (Interleukin 12 alpha (IL12A))
    Reaktivität
    • 58
    • 13
    • 6
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    Maus
    Wirt
    • 51
    • 14
    • 5
    • 2
    • 2
    Ziege
    Klonalität
    • 58
    • 16
    Polyklonal
    Konjugat
    • 43
    • 10
    • 7
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser IL12A Antikörper ist konjugiert mit Biotin
    Applikation
    • 56
    • 31
    • 30
    • 9
    • 8
    • 7
    • 7
    • 7
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Spezifität
    This antibody detects Interleukin-12.
    Aufreinigung
    Affinity chromatography
    Immunogen
    Highly pure (> 98 %) recombinant murine IL-12
    Top Product
    Discover our top product IL12A Primärantikörper
  • Applikationshinweise
    ELISA: Direct: To detect mIL-12 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-12. Sandwich: To detect mIL-12 (using 100 μL/well antibody solution) a concentration of0.25 - 1.0 μg/mL of this antibody is required. In conjunction with Polyclonal Anti-MurineIL-12 as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant mIL-12. Western blot: To detect mIL-12 this antibody can be used at a concentration of0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detectionlimit for recombinant mIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducingconditions.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Rekonstitution
    Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 -1 .0 mg/mL.
    Buffer
    PBS, pH 7.2
    Handhabung
    This product is photosensitive and should be protected from light
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
  • Target
    IL12A (Interleukin 12 alpha (IL12A))
    Andere Bezeichnung
    Interleukin-12 alpha / IL12A (IL12A Produkte)
    Synonyme
    CLMF antikoerper, IL-12A antikoerper, NFSK antikoerper, NKSF1 antikoerper, P35 antikoerper, CLMF2 antikoerper, IL-12B antikoerper, NKSF antikoerper, NKSF2 antikoerper, p40 antikoerper, Il-12b antikoerper, Il12p40 antikoerper, Il-12p40 antikoerper, Il12 antikoerper, IL-12p40 antikoerper, IL-12p35 antikoerper, Il-12a antikoerper, Ll12a antikoerper, p35 antikoerper, IL12p35 antikoerper, Il-12 p35 antikoerper, IL12p40 antikoerper, IL12 antikoerper, IL-12 p35 antikoerper, interleukin 12A antikoerper, interleukin 12B antikoerper, interleukin 12b antikoerper, interleukin 12a antikoerper, Interleukin 12 level antikoerper, IL12A antikoerper, IL12B antikoerper, Il12b antikoerper, Il12a antikoerper, IL12 antikoerper, il12a antikoerper
    Hintergrund
    Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells, costimulation of PBL proliferation, proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10, homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35.Synonyms: CLMF p35, Cytotoxic lymphocyte maturation factor 35 kDa subunit, IL-12 subunit p35, IL-12A, NK cell stimulatory factor chain 1, NKSF1
    Gen-ID
    16159
    NCBI Accession
    NP_032377
    UniProt
    P43431
    Pathways
    JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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