beta-Catenin (CTNNB1) Antikörper

Antigen: beta-Catenin (CTNNB1)

Synonyme: CTNNB, FLJ25606, FLJ37923, DKFZp686D02253, Mesc, Catnb, ctnnb, id:ibd2058, wu:fb73e10, wu:fi81c06, wu:fk25h01, ARM, Beta-cat, Beta-catenin, EG:86E4.6, beta-cat-arm, l(1)2Bv, l(1)G0192, l(1)G0234, l(1)G0410, l(1)arm, t12687 ALR Dm, DmelCG11579, CG11579, beta-catenin, ctnnb1, MGC79992, MGC132060, Cibeta-catenin, CTNNB1, MGC52601, LOC576711, Ctnnb1, MGC139922

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Huhn, Human, Maus, Ratte (Rattus), Xenopus laevis

Applikation: Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN181394

Produktbeschreibung

Produktmerkmale: Synonyms: CTNNB1, CTNNB, beta-1 Catenin, beta catenin, beta-Catenin 1, Catenin-beta 1

Weitere Bezeichnung: Catenin beta-1

Swiss-Prot: P35222

Immunogen: A synthetic peptide from the C-terminus of the β-catenin protein. Remarks: Mol. Wt. of Antigen: 92 kDa

Beschreibung: The catenins (α, β and γ) are ubiquitously expressed, cytoplasmic proteins associated withE-cadherin at cellular junctions. β- catenin also binds to N-cadherin andcoimmunoprecipitates with APC. Cadherin/catenin complexes are linked to thecytoskeleton via a direct association between α-actinin and α-catenin. Increased tyrosinephosphorylation can disrupt catenin-cadherin complexes, influencing cellular adhesion.

Spezifität: This antibody detects Catenin-beta. Cellular Localization: Cytoplasmic and cell membraneSpecies: Human, Mouse, Rat, Chicken and Xenopus. Others not tested.

Synonyme: CTNNB, FLJ25606, FLJ37923, DKFZp686D02253, Mesc, Catnb, ctnnb, id:ibd2058, wu:fb73e10, wu:fi81c06, wu:fk25h01, ARM, Beta-cat, Beta-catenin, EG:86E4.6, beta-cat-arm, l(1)2Bv, l(1)G0192, l(1)G0234, l(1)G0410, l(1)arm, t12687 ALR Dm, DmelCG11579, CG11579, beta-catenin, ctnnb1, MGC79992, MGC132060, Cibeta-catenin, CTNNB1, MGC52601, LOC576711, Ctnnb1, MGC139922

Anwendungen

Applikationshinweise: Immunoprecipitation (Native verified): Use Protein A, Ab 10 g/mg protein lysate. Western Blotting: 5-10 ug/ml for 2 hrs at RT. Immunohistology (Formalin/paraffin): Use Ab at 1-2 ug/ml for 30 min at RT. [Staining offormalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0), for10-20 min followed by cooling at RT for 20 min. ]Positive Control: MCF-7 cells. Breast carcinoma. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Buffer: 10 mM PBS, pH 7.4, with 0.2 % BSA & 0.09 % sodium azide.

Lagerung: Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.

Forschungsgebiet: Signaltransduktion

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Formalin-fixed, paraffin-embedded human breast cancer stained with Catenin,beta (ABIN181394) using peroxidase-conjugate and AEC chromogen. Note cytoplasmic and membrane staining of tumor cells.