X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 2 (XRCC1) Antikörper

Antigen: X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 2 (XRCC1)

Synonyme: DKFZp781P0919, RecA, RAD51, 4921524O04Rik, 8030409M04Rik, XRCC2, RGD1564823, MGC160004

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN181348

Produktbeschreibung

Produktmerkmale: Synonyms: DNA repair protein XRCC2, X-ray repair cross-complementing protein 2

Weitere Bezeichnung: XRCC2

Swiss-Prot: O43543

Immunogen: A synthetic peptide derived from the N-terminus of human XRCC2 protein. Remarks: Mol. Wt. of Antigen: 32 kDa

Beschreibung: XRCC2 (X-Ray Cross Complementing 2) is essential for repairing the double srand DNAbreaks by homologous recombination between sister chromatids. It is homologous toRad51. Five human homologues of RAD 51, XRCC2, XRCC3, RAD51B, RAD51C, RAD51D,interact among themselves to form one big complex or several smaller complexes.

Spezifität: This antibody detects XRCC2. Cellular Localization: Nuclear and cytoplasmicSpecies: Human. Others not tested.

Anwendungen

Applikationshinweise: Western Blotting: Ab 5-10 ug/ml for 2 hrs at RT. Immunohistology (formalin/paraffin): Ab 10-20 ug/ml for 30 min at RT. Staining offormalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for10-20 min followed by cooling at RT for 20 min. Recommended positive control: HT29 orHeLa cells. Testis. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1 mg/ml

Buffer: 10 mM PBS, pH 7.4, with 0.2 % BSA & 0.09 % sodium azide.

Lagerung: Store the antibody at 4-8 °C for one month or at -20 °C for longer. Avoid repeated freezingand thawing. Shelf life: one year from despatch.

Forschungsgebiet: Chromatin und nukleäre Signaltransduktion, DNS/RNS

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Formalin-fixed, paraffin-embedded human testis stained with XRCC2 (ABIN181348) using peroxidase-conjugate and AEC chromogen. Note nuclear staining of germ cells.