E2F5 Antikörper
| Antigen |
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| Klonalität |
Polyklonal |
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Wirt
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Reaktivität
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Applikation
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 Alternativen Elektromobilitäts-Experimente (EMSA), Western Blot (WB)
Durchflusszytometrie (FACS) (18), Immunfluoreszenz (IF) (18), Western Blot (WB) (10), ELISA (3), Immunpräzipitation (IP) (3), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunhistochemie (Paraffinschnitte) (IHC (p)) (2), Dot Blot (Dot) (1), Enzyme Immunoassay (EIA) (1), Immunzytochemie (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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| Produktnummer |
ABIN181326 |
| Menge |
0.5 mg (1.0 mg/ml) (Varianten) |
| Preis |
380,00 € Zzgl. Versandkosten €40,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
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Produktmerkmale
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Synonyms: E2F-5, Transcription factor E2F5, E2F transcription factor 5, p130-binding
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Swiss-Prot
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Q15329
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Immunogen
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Synthetic peptide from the N-terminus of human E2F-5 protein. Remarks: Mol. Wt. of Antigen: ~58 kDa
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Beschreibung
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E2F transcription factors are functionally regulated by binding to Rb p110, p107, and p130. E2F family members bind DNA as heterodimers with members of the DP family ofpolypeptides.
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Spezifität
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This antibody detects E2F-5 Transcription Factor. Species: Human, Mouse, and Rat. Others not tested.
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Applikationshinweise
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Gel Supershift Assay. Western Blotting (5-10g/ml for 2hrs at RT, Recommended positive control: MCF-7, NIH/3T3Cells). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Konzentration
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1.0 mg/ml
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Buffer
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10 mM PBS, pH 7.4, with 0.2 % BSA & 0.09 % sodium azide.
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Lagerung
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Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.
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Forschungsgebiet
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Chromatin und nukleäre Signaltransduktion, Transkriptionsfaktoren
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "E2F5", Typ "Antikörper" finden
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Wirte
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Kaninchen (26), Maus (3)
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Reaktivitäten
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Human (29), Maus (23), Ratte (Rattus) (22), Rind (Kuh) (2), Huhn (1), Hund (1), Zebrafisch (Brachydanio rerio) (1)
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Applikationen
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Durchflusszytometrie (FACS) (18), Immunfluoreszenz (IF) (18), Western Blot (WB) (10), ELISA (3), Immunpräzipitation (IP) (3), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunhistochemie (Paraffinschnitte) (IHC (p)) (2), Dot Blot (Dot) (1), Enzyme Immunoassay (EIA) (1), Immunzytochemie (ICC) (1), Immunoelectron Microscopy (IEM) (1)
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Konjugate
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitope
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AA 9-27 (1)
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