beta-Catenin (CTNNB1) Antikörper

Antigen: beta-Catenin (CTNNB1)

Synonyme: CTNNB, FLJ25606, FLJ37923, DKFZp686D02253, Mesc, Catnb, ctnnb, id:ibd2058, wu:fb73e10, wu:fi81c06, wu:fk25h01, ARM, Beta-cat, Beta-catenin, EG:86E4.6, beta-cat-arm, l(1)2Bv, l(1)G0192, l(1)G0234, l(1)G0410, l(1)arm, t12687 ALR Dm, DmelCG11579, CG11579, beta-catenin, ctnnb1, MGC79992, MGC132060, Cibeta-catenin, CTNNB1, MGC52601, LOC576711, Ctnnb1, MGC139922

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Hund, Human, Maus

Applikation: Immunfluoreszenz (IF), Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN181305

Produktbeschreibung

Produktmerkmale: Synonyms: CTNNB1, CTNNB, beta-1 Catenin, beta catenin, beta-Catenin 1, Catenin-beta 1

Weitere Bezeichnung: Catenin beta-1

Swiss-Prot: P35222

Immunogen: Recombinant Human beta-Catenin protein.

Beschreibung: The catenins (alpha, beta and gamma ) are ubiquitously expressed, cytoplasmic proteinsassociated with E-cadherin at cellular junctions. Alpha catenin also binds to N-cadherinand coimmunoprecipitates with APC. Cadherin/catenin complexes are linked to thecytoskeleton via a direct association between alpha actinin and alpha catenin. Increasedtyrosine phosphorylation can disrupt catenin-cadherin complexes, influencing cellularadhesion.

Spezifität: Highly specific for Catenin beta. Cellular Localization: Cytoplasmic, Cell Membrane. Species Reactivity: Tested: Human and Mouse. Expected from sequence similarity: Canine (Dog). Add. Information: Mol. Wt. of Antigen: 92kD.

Synonyme: CTNNB, FLJ25606, FLJ37923, DKFZp686D02253, Mesc, Catnb, ctnnb, id:ibd2058, wu:fb73e10, wu:fi81c06, wu:fk25h01, ARM, Beta-cat, Beta-catenin, EG:86E4.6, beta-cat-arm, l(1)2Bv, l(1)G0192, l(1)G0234, l(1)G0410, l(1)arm, t12687 ALR Dm, DmelCG11579, CG11579, beta-catenin, ctnnb1, MGC79992, MGC132060, Cibeta-catenin, CTNNB1, MGC52601, LOC576711, Ctnnb1, MGC139922

Anwendungen

Applikationshinweise: Immunofluorescence. Immunoprecipitation (native): 10 µg/mg protein lysate, use Protein A. Western Blotting (2.5-5 µg/ml for 2 hrs at RT). Immunohistology (Formalin/Paraffin): 10-20 µg/ml for 30 min at RT. Staining offormalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0). Recommended Positive Control: MCF-7 Cells, Breast Carcinoma. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Buffer: 10 mM PBS, pH 7.4, with 0.09% Sodium Azide as preservative and 0.2%BSA as stabilizer.

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Forschungsgebiet: Signaltransduktion

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Formalin-fixed, paraffin-embedded human breast carcinoma stained with Catenin beta Ab (Cat. ) using peroxidase-conjugate and AEC chromogen. Note membrane staining of tumor cells.