TGFB1 Antikörper

Produktnummer ABIN181152

Produktdetails anti-TGFB1 Antikörper

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TGFB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Spezifität: This antibody detects TGF-3.

Produktmerkmale: Synonyms: Transforming growth factor beta-1, TGFB, LAP, Latency-associated peptide

Aufreinigung: Affinity Chromatography on Protein G

Immunogen: Highly pure (>98%) recombinant Human TGFB1

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: ELISA: In a sandwich ELISA (assuming 100μL/well), a concentration of 4.0-8.0 μg/mL of thisantibody will detect at least 100 pg/mL of recombinant Human TGFB1 when used inconjunction with compatible secondary reagents. Western Blot: To detect Human TGFB1 by Western Blot analysis this antibody can be usedat a concentration of 2.0-4.0 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human TGFB1 is 2.0-4.0 ng/lane, undernon-reducing conditions. In a dot blot, a 1/500 dilution of this antibody exhibited complete cross reactivity withHuman TGFB2 and TGFB3 isotypes. Reduced cross reactivity was shown at a 1/1000dilution.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2-8 °C for six weeks. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu TGFB1

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Andere Bezeichnung: TGFB1 (TGFB1 Produkte)

Hintergrund: Synonyms: TGF-beta-1, TGFB, Transforming growth factor beta-1

Gen-ID: 7040

UniProt: P01137

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie, Cancer Immune Checkpoints