Substance P Antikörper
| Antigen |
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| Synonyme |
NK2, NPK, NKNA, TAC2, Hs.2563, SP, NK1, NK-1, Nkna, PPT-A, MGC151458, 4930528L02Rik |
| Klonalität |
Monoklonal (NC1-34) |
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Wirt
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Reaktivität
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Human (39), Ratte (Rattus) (26), Maus (22), Meerschweinchen (17), Rind (Kuh) (16), Kaninchen (16), Pferd (15), Schaf (15), Säugetiere (2), Huhn (1), Fisch (1), Affe (1), Schwein (1)
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Applikation
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Alternativen Immunhistochemie (Gefrierschnitte) (IHC (fro)), Immunfluoreszenz (IF), Immunhistochemie (Paraffinschnitte) (IHC (p))
Immunfluoreszenz (IF) (31), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (20), Durchflusszytometrie (FACS) (19), Immunhistochemie (Paraffinschnitte) (IHC (p)) (16), ELISA (8), Immunhistochemie (IHC) (8), Enzyme Immunoassay (EIA) (4), Western Blot (WB) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunpräzipitation (IP) (2), Immunoelectron Microscopy (IEM) (1), Radioimmunoassay (RIA) (1)
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| Produktnummer |
ABIN181043 |
| Menge |
0.1 ml |
| Preis |
430,00 € Zzgl. Versandkosten €40,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
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Produktmerkmale
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Synonyms: Substance-P
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Gen-ID
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6863
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Swiss-Prot
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P20366
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Immunogen
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Substance P conjugated to Bovine Serum Albumin. Spleen cells from immunised Wistar ratwere fused with cells of the NS1/1-Ag 4-1 myeloma cell line.
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Format
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Supernatant
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Isotyp
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IgG2a (Passende Sekundärantikörper)
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Klon
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NC1-34
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Beschreibung
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Substance P is known to have a role in several physiologic activities such as paintransmission, the vomiting reflex, salivary secretion and smooth muscle contraction. 5%reactivity is observed with eledoisin. It does not react with Leu or Met-enkephalin,somatostatin or betaendorphin.
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Spezifität
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This antibody recognizes the COOH terminal end of substance P, a short polypeptideneurotransmitter that regulates the excitability of dorsal horn nociceptive neurons. 5 % reactivity is observed with eledoisin. It does not react with Leu or Met-enkephalin,somatostatin or beta-endorphin.
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Applikationshinweise
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Immunohistochemistry on frozen sections: 1/100 - 1/200. Immunohistochemistry on paraffin sections: 1/100 - 1/200, Pre-treatment is not required. Immunofluorescence: This antibody has been used for the localisation of substance P intissue sections by immunofluorescence and PAP staining in nervous systems ofexperimental animals and man. Please refer to the Cuello references listed below. It hasalso been successfully applied in archive post-mortem tissues of the human brain, see Maiand Pioro references. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Buffer
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0.09% Thiomersal
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Lagerung
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Forschungsgebiet
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Neurologie, Zell-/Gewebemarker
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Substance P", Typ "Antikörper" finden
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Wirte
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Kaninchen (39), Maus (9), Meerschweinchen (4), Ratte (2)
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Reaktivitäten
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Human (39), Ratte (Rattus) (26), Maus (22), Meerschweinchen (17), Rind (Kuh) (16), Kaninchen (16), Pferd (15), Schaf (15), Säugetiere (2), Huhn (1), Fisch (1), Affe (1), Schwein (1)
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Applikationen
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Immunfluoreszenz (IF) (31), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (20), Durchflusszytometrie (FACS) (19), Immunhistochemie (Paraffinschnitte) (IHC (p)) (16), ELISA (8), Immunhistochemie (IHC) (8), Enzyme Immunoassay (EIA) (4), Western Blot (WB) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunpräzipitation (IP) (2), Immunoelectron Microscopy (IEM) (1), Radioimmunoassay (RIA) (1)
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Konjugate
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitope
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AA 4-11 (2)
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