Microtubule Associated Protein 2 (MAP2) Antikörper

Antigen: Microtubule Associated Protein 2 (MAP2)

Synonyme: MAP2A, MAP2B, MAP2C, DKFZp686I2148, MAP2, MAP-2, Mtap-2, xmap2, MGC53131, a730034c02, map2, MGC145864

Klonalität: Monoklonal (AP18)

Wirt: Maus

Reaktivität: Rind (Kuh), Human, Ratte (Rattus), Xenopus laevis

Applikation: Immunfluoreszenz (IF), Immunhistochemie (Paraffinschnitte) (IHC (p)), Western Blot (WB)

Produktnummer: ABIN180702

Produktbeschreibung

Produktmerkmale: Synonyms: Microtubule-associated protein 2, MAP2, Neuronal Marker

Weitere Bezeichnung: MAP-2

Gen-ID: 4133

Swiss-Prot: P11137

Immunogen: Bovine brain microtubule associated protein 2Remarks: Mol. Wt. of Antigen: ~280 kDa Doublet and a 70 kDa singlet

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: AP18

Beschreibung: Microtubule Associated Protein 2 exists in two high molecular weight forms (2a & 2b) and alow molecular weight form. The expression of MAP2 is developmentally regulated and itsmultiple forms arise by alternative splicing of a single gene.

Spezifität: This antibody is specific to phosphprylated form of MAP2a,b,c and shows nocross-reaction with other MAPs, tau, and tubulin. It reacts with dendrites and cell bodies ofneurons. It is useful in studies of neuron structure in normal and malignantbrain tissue andin Alzheimer's. Species: Human, Cow, Rat, Xenopus, Quail. Others not tested. Add. Information: Cellular Localization: CytoplasmicEpitope: aa 1-151

Anwendungen

Applikationshinweise: Immunofluorescence. Western Blotting (1,2): 1-2 ug/ml for 2 hrs at RT. Immunohistology (Formalin/paraffin): 1-2 ug/ml for 30 min at RT, staining of formalin-fixedtissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minfollowed by cooling at RT for 20 min. Positive Control: IMR-5 or T98G human glioblastoma cells or brain. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 0.2 mg/ml

Buffer: 10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide

Lagerung: Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.

Forschungsgebiet: Zytoskelett

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Formalin-fixed, paraffin-embedded human brain stained with Microtubule-Associated Protein 2a,b,c (ABIN180702) using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of neurons and dendrites.