Estrogen Receptor (ER) Antikörper

Antigen: Estrogen Receptor (ER)

Synonyme: LOC398733, LOC398734, ERa, ESR, Estr, ER[a], Estra, Nr3a1, ERalpha, AA420328, AU041214, ER-alpha, ESR1, NR3A1, eralpha, zfER[a], Errra, ERRalpha, ESRRA, err1, erra, esrral, erralpha, LOC100125507, ESTRRA, esrl1, nr3b1, MGC147343, ERRa, ER[b]1, zfER[b]1, MGC103724, zfER-beta1, erb2, erb1, CERB, ESR2, ERB, erb, esrb, estrb, nr3a2, ERbeta, er-beta, esr-beta, ERBETA, er, ER-beta, ER[b], Estrb, ER[b]2, ER[b]a, zfER[b]2, zfER-beta2, LOC100125508, errb2, ER, Era, ESRA, DKFZp686N23123, 7B4, CD144, FLJ17376, ACTGP1, ACT1GP1, HY-psi-gamma-AC6, Esr, RNESTROR, ERALPHA, esr, esr1, nr3a1, AAC2, PNAT, XER, era, esra, xesr-1, xlERalpha1, xlERalpha2

Klonalität: Monoklonal (TE111-5D11)

Wirt: Maus

Reaktivität: Rind (Kuh), Hund, Huhn, Hamster, Human, Maus, Schwein, Kaninchen, Ratte (Rattus), Schaf

Applikation: Elektromobilitäts-Experimente (EMSA), Funktionale Studien (Func), Immunpräzipitation (IP), Western Blot (WB)

Produktnummer: ABIN180472

Produktbeschreibung

Produktmerkmale: Synonyms: ER-alpha, ER-beta, ER, ESR, ESR1, ESR2, ESRA, ESRB, ERA, NR3A1, Estradiol Receptor

Weitere Bezeichnung: Estrogen Receptor

Swiss-Prot: P03372

Immunogen: 35 kDa, C-terminal fragment (302-553) of Human ER expressed in E. coli. Remarks: Mol. Wt. of Antigen: 67 kDaEpitope: aa 302-553.

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: TE111-5D11

Beschreibung: ER gene consists of more than 140 kb of genomic DNA divided into 8 exons, beingtranslated into a protein with six functionally discrete domains required for transcriptionactivation function, binding to estrogen response element (ERE) constitutive dimerization,binding to heat shock proteins, and ligand recognition. The ER is an important regulator ofgrowth and differentiation in the mammary gland. Presence of ER in breast tumorsindicates an increased likelihood of response to anti-estrogen (e. g. tamoxifen) therapy.

Spezifität: This antibody detects Estrogen Receptor. Cellular Localization: Nuclear. Species: Human, Cow, Pig, Dog, Mouse, Rat, Sheep, Rabbit, Hamster, and Chicken. Others not tested.

Synonyme: LOC398733, LOC398734, ERa, ESR, Estr, ER[a], Estra, Nr3a1, ERalpha, AA420328, AU041214, ER-alpha, ESR1, NR3A1, eralpha, zfER[a], Errra, ERRalpha, ESRRA, err1, erra, esrral, erralpha, LOC100125507, ESTRRA, esrl1, nr3b1, MGC147343, ERRa, ER[b]1, zfER[b]1, MGC103724, zfER-beta1, erb2, erb1, CERB, ESR2, ERB, erb, esrb, estrb, nr3a2, ERbeta, er-beta, esr-beta, ERBETA, er, ER-beta, ER[b], Estrb, ER[b]2, ER[b]a, zfER[b]2, zfER-beta2, LOC100125508, errb2, ER, Era, ESRA, DKFZp686N23123, 7B4, CD144, FLJ17376, ACTGP1, ACT1GP1, HY-psi-gamma-AC6, Esr, RNESTROR, ERALPHA, esr, esr1, nr3a1, AAC2, PNAT, XER, era, esra, xesr-1, xlERalpha1, xlERalpha2

Anwendungen

Applikationshinweise: Gel Supershift. Immunoprecipitation (Native and denatured): Use Protein G, 2 µg/mg protein lysate. Western Blotting: 1-2 µg/ml for 2hrs at RT. Blocks binding of Estrogen to ER. Chromatin Precipitation. Positive Control: T47D cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Buffer: 10 mM PBS, pH 7.4 without BSA and Azide.

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Forschungsgebiet: Krebs, Endokrines System, Transkriptionsfaktoren, Hormone, Rezeptoren

Beschränkungen: Nur für Forschungszwecke einsetzbar