E2F3 Antikörper
| Antigen |
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| Klonalität |
Monoklonal (PF37) |
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Wirt
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Reaktivität
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Applikation
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 Alternativen Elektromobilitäts-Experimente (EMSA), Immunfluoreszenz (IF)
Immunfluoreszenz (IF) (26), Durchflusszytometrie (FACS) (18), Western Blot (WB) (17), Immunpräzipitation (IP) (8), Gel Shift (GS) (7), ELISA (6), Enzyme Immunoassay (EIA) (4), Immunhistochemie (Paraffinschnitte) (IHC (p)) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Dot Blot (Dot) (1), Elektromobilitäts-Experimente (EMSA) (1), Immunzytochemie (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunhistochemie (IHC) (1)
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| Produktnummer |
ABIN180471 |
| Menge |
0.1 mg (0.2 mg/ml) (Varianten) |
| Preis |
460,00 € Zzgl. Versandkosten €40,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
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Produktmerkmale
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Synonyms: E2F-3, KIAA0075, MGC104598, DKFZp686C18211, Transcription factor E2F3, E2Ftranscription factor 3
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Swiss-Prot
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O00716
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Immunogen
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Recombinant human E2F-3 protein.
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Isotyp
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IgG2a (Passende Sekundärantikörper)
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Klon
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PF37
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Beschreibung
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E2F transcription factors are functionally regulated by binding to Rb p110, p107, and p130. E2F-3 is regulated by complex formation with Rb p110. E2F family members bind DNA asheterodimers with members of the DP family of polypeptides.
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Spezifität
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This antibody detects 58kDa E2F3 protein. No reactivity with other related E2F transcriptionfactors. Cellular Localization: Nuclear and cytoplasmic. Species: Human. Others not tested.
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Applikationshinweise
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Gel Supershift (use 1 mg/ml Cat. No ABIN180470). Immunofluorescence. Positive Control: Raji cells. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Konzentration
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0.2 mg/ml
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Buffer
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10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide.
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Lagerung
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Store the antibody at 2 - 8 °C up to one month or in aliquots at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.
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Forschungsgebiet
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Chromatin und nukleäre Signaltransduktion, Transkriptionsfaktoren
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "E2F3", Typ "Antikörper" finden
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Wirte
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Kaninchen (25), Maus (18)
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Reaktivitäten
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Human (40), Maus (21), Ratte (Rattus) (18), Rind (Kuh) (16), Hund (16), Pferd (15), Schwein (15), Kaninchen (15)
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Applikationen
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Immunfluoreszenz (IF) (26), Durchflusszytometrie (FACS) (18), Western Blot (WB) (17), Immunpräzipitation (IP) (8), Gel Shift (GS) (7), ELISA (6), Enzyme Immunoassay (EIA) (4), Immunhistochemie (Paraffinschnitte) (IHC (p)) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Dot Blot (Dot) (1), Elektromobilitäts-Experimente (EMSA) (1), Immunzytochemie (ICC) (1), Immunoelectron Microscopy (IEM) (1), Immunhistochemie (IHC) (1)
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Konjugate
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitope
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AA 70-82 (1)
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