E2F1 Antikörper
| Antigen |
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| Klonalität |
Monoklonal (KH20) |
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Wirt
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Reaktivität
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Applikation
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 Alternativen Elektromobilitäts-Experimente (EMSA), Immunfluoreszenz (IF)
Western Blot (WB) (63), Immunfluoreszenz (IF) (36), Immunpräzipitation (IP) (28), Immunhistochemie (Paraffinschnitte) (IHC (p)) (19), Durchflusszytometrie (FACS) (18), Enzyme Immunoassay (EIA) (13), ELISA (9), Gel Shift (GS) (8), Immunhistochemie (IHC) (5), Dot Blot (Dot) (3), Immunzytochemie (ICC) (3), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (2), Immunoelectron Microscopy (IEM) (1)
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| Produktnummer |
ABIN180411 |
| Menge |
0.1 mg (0.2 mg/ml) |
| Preis |
380,00 € Zzgl. Versandkosten €40,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
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Produktmerkmale
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Synonyms: E2F-1, RBBP3, PBR3, Transcription factor E2F1, Retinoblastoma-binding protein 3,PRB-binding protein E2F-1, Retinoblastoma-associated protein 1, E2F transcription factor 1
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Swiss-Prot
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Q01094
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Immunogen
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Recombinant Human E2F-1 protein. AA Sequence: 1-89Remarks: Mol. Wt. of Antigen: ~60 kDa
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Isotyp
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IgG2a (Passende Sekundärantikörper)
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Klon
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KH20
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Beschreibung
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E2F's are DNA-binding proteins that associate with negative regulators, such as theretinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F-1 alsorequires DP-1 for efficient DNA-binding and transcription modification. E2F1 is proposed tobe involved in several cellular processes that range from tumor suppression, cell cycleprogression, and oncogenesis. E2F-1 overexpression can also drive cells into apoptosis. These observations place E2F among a group of apoptosis inducers that includes, c-Myc,Adenovirus E1A, and the human papilloma virus E7 protein.
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Spezifität
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Thus antibody detects E2F-1 Transcription Factor. Cellular Localization: Nuclear. Species: Human, Mouse, Rat. Others not tested.
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Applikationshinweise
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Gel Supershift: use Ab at 1mg/ml. Immunofluorescence. Recommended positive control: MAD109 and HL-60 Cells. Not suitable for Immunohistology, Immunoprecipitation and Western Blotting. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Konzentration
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0.2 mg/ml
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Buffer
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10 mM PBS, pH 7.4, with 0.2 % BSA and 0.09 % sodium azide.
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Lagerung
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Store the antibody at 2 - 8 °C for one month or in aliquots at -20 °C for longer. Avoidrepeated freezing and thawing. Shelf life: one year from despatch.
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Forschungsgebiet
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Krebs, Transkriptionsfaktoren
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "E2F1", Typ "Antikörper" finden
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Wirte
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Kaninchen (56), Maus (33)
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Reaktivitäten
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Human (85), Maus (30), Ratte (Rattus) (22), Rind (Kuh) (4), Hund (4), Huhn (3), Schwein (3), Kaninchen (3), Pferd (2)
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Applikationen
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Western Blot (WB) (63), Immunfluoreszenz (IF) (36), Immunpräzipitation (IP) (28), Immunhistochemie (Paraffinschnitte) (IHC (p)) (19), Durchflusszytometrie (FACS) (18), Enzyme Immunoassay (EIA) (13), ELISA (9), Gel Shift (GS) (8), Immunhistochemie (IHC) (5), Dot Blot (Dot) (3), Immunzytochemie (ICC) (3), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (2), Immunoelectron Microscopy (IEM) (1)
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Konjugate
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Biotin (4), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitope
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pSer364 (2), C-Term (1), pSer332 (1), pSer337 (1), pThr433 (1)
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