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Antikörper
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anti-Flotillin 1 (FLOT1) (C-Term) Antikörper
Flotillin 1 (FLOT1) (C-Term) Antikörper
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| Synonyme |
FLOT1, DKFZp469E0515, flot1, Rareg, flot1c, MGC84970, MGC132244, flotillin1, flot1a, flotillin-1, MGC143379, reggie2, wu:fj45g11, si:dz44o19.1 |
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Epitope
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| Klonalität |
Polyklonal |
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Wirt
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Reaktivität
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Applikation
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| Produktnummer |
ABIN180228 |
| Menge |
100 µg |
| Preis |
324,00 € Zzgl. Versandkosten €40,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 10 bis 15 Werktagen |
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Weitere Bezeichnung
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Flotillin-1 (C)
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Immunogen
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Synthetic peptide of common part of C terminal of rat and mouse Flotillin-1 (SQVNHNKPLRTA) Purification: Purified with antigen peptide
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Isotyp
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IgG (Passende Sekundärantikörper)
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Beschreibung
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Raft is rich in sphingo-gliycolipids and cholesterol, it is advocated as a membrane domain which participates in protein sorting, and it is known that many signal transduction molecules are gathering there. It is difficult to distinguish catch and identify Raft protein, unlike Cabeole which is the another micro domain of membrane, because the protein moves continuously on a cell membrane, and because the size and the structure of the protein are labile. For this reason, the marker specific to Raft was required in order to identify. Flotillin is the composition ingredient of Raft and the possibility as a marker of Raft is suggested.
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Spezifität
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Reacts with Rat, Mouse and Human Flotillin-1 (Confirmed by western blotting and Immunohistochemistry)
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Applikationshinweise
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This antibody can be stained in formalin fixed paraffin embedded tissues after microwave pretreatment (10 min, 1-0mM Citrate Buffer, pH 6.0) by several Immunohistochemical techniques such as Avidin Biotin Complex (ABC) method. The optimal dilution is about 2 ug/ml, however, the dilution rate should be optimized by each laboratories.:This antibody can be used for western blotting in concentration of about 2 ug/ml.
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Reinigung
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Antigen peptide
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Buffer
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Lyophilized product from 1% BSA in PBS containing 0.05% NaN3 . Reconstitution: 1.0 ml distilled water will be added to the product, then its concentration comes to 100 ug/ml
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Lagerung
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Lyophilized product, 5 years at 2 8 : Solution, 2 years at 20
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Flotillin 1 (FLOT1)", Typ "Antikörper" finden
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Wirte
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Kaninchen (25), Ziege (6), Maus (1)
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Reaktivitäten
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Human (29), Maus (20), Ratte (Rattus) (17), Rind (Kuh) (7), Hund (7), Schwein (6), Kaninchen (4), Pferd (2), Meerschweinchen (1), Schaf (1)
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Applikationen
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Durchflusszytometrie (FACS) (17), Immunfluoreszenz (IF) (16), Western Blot (WB) (13), ELISA (3), Enzyme Immunoassay (EIA) (3), Immunhistochemie (Paraffinschnitte) (IHC (p)) (2), ELISA (Detektionsantikörper) (1), Immunoelectron Microscopy (IEM) (1), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (1), Immunpräzipitation (IP) (1)
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Konjugate
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Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitope
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AA 365-427 (1), C-Term (1)
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