Cre Recombinase Antikörper

Antigen: Cre Recombinase

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: Western Blot (WB), Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN180208

Produktbeschreibung

Immunogen: Synthetic peptide for middle portion of Cre recombinase (MLHRRSGLPRPSDSNAV) Purification: Affinity purified with synthetic peptide

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Cre is a 38 kDa recombinase protein from bacteriophage P1 which mediates intramolecular and intermolecular site specific recombination between loxP sites. The role of Cre is to resolve dimers of P1 that arise after replication in order to allow partitioning of the two P1 molecules at cell division. A loxP site (locus of X-ing over) consists of two 13 bp inverted repeats separated by an 8 bp asymmetric spacer region. One molecule of Cre binds per inverted repeat or two Cre molecules line up at one loxP site. The recombination occurs in the asymmetric spacer region. Those 8 bases are also responsible for the directionality of the site. Two loxP sequences in opposite orientation to each other invert the intervening piece of DNA, two sites in direct orientation dictate excision of the intervening DNA between the sites leaving one loxP site behind. Thus, this precise removal of DNA can be used to eliminate an endogenous gene or transgene activate a transgene.

Spezifität: Confirmed by western blotting using Cre Cos/TNE sup.

Anwendungen

Applikationshinweise: This antibody can be used for immunohistochemistry with formalin fixed paraffin embedded tissues after microwave treatment (in 10 mM citrate buffer, ph 6.0, for 10 min.) by several techniques such as Avidin Biotin Complex (ABC) Method. The optimal concentration is about 2-5 μg/mL, however, the concentration should be optimized by each laboratory. This antibody can be used for western blotting in concentration of 1-3 μg /mL.

Reinigung: Purified with antigen peptide

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05%NaN3. Reconstitution: 1.0 mL deionized water will be added to the product, then its concentration comes to 50 g/mL.

Lagerung: Lyophilized product, 5 years at 2 ­ 8 °C : Solution, 2 years at ­20 °C

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Cre Recombinase antibody

Brain

anti-Cre Recombinase antibody (Image 3)