Stratifin (SFN) (C-Term) Antikörper

Antigen: Stratifin (SFN)

Synonyme: YWHAS, Er, Mme1, Ywhas, LOC408037, LOC100220844

Epitope: C-Term

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunhistochemie (IHC), Western Blot (WB)

Produktnummer: ABIN180188

Produktbeschreibung

Weitere Bezeichnung: 14-3-3 sigma Protein (C)

Immunogen: Synthetic peptide of c-terminus portion for human 14-3-3 Sigma protein Purification: Affinity purified with synthetic peptide

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: The cell cycle checkpoint plays an important role in maintaining the integrity of cells. The G1 arrest is due to p53-mediated induction of the cyclin-dependent kinase inhibitor p21WAF1/CIP1/SDI1, but the basis for the G2 arrest is unknown. Recently, one of the 14-3-3 protein family members, 14-3-3sigma, was shown to be regulated by p53 and to play a role in the G2-M-phase checkpoint. 14-3-3 sigma was cloned by expression cloning through cyclin-dependent kinase 2 (CDK2) association. 14-3-3 sigma shares cyclin-CDK2 binding motifs with different cell cycle regulators, including p107, p130, p21 (CIP1), p27 (KIP1), and p57 (KIP2), and is associated with cyclin.

Spezifität: Human 14-3-3 Sigma specific. No crossreactivities with 14-3-3 Beta, Gamma, Epsilon, Zeta, Eta, and Tau. (Confirmed by western blotting)

Anwendungen

Applikationshinweise: This antibody can be stained in formalin fixed paraffin embedded tissues after microwave treatment (10 Min, 1-0mM Citrate Buffer, pH 6.0) by several Immunohistochemical techniques such as Avidin Biotin Complex (ABC) Method. The optimal dilution is about 1 - 3 ug/ml, however, the dilution rate should be optimized by each laboratories.:This antibody can be used for western blotting in concentration of 1 - 3 ug/ml.

Reinigung: Antigen peptide

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 . Reconstitution: 1.0 ml distilled water will be added to the product

Lagerung: Lyophilized product, 5 years at 2 ­ 8 C : Solution, 2 years at ­20 C

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

BALB C Mouse Skin

anti-Stratifin (SFN) (C-Term) antibody (Image 2)

anti-Stratifin (SFN) (C-Term) antibody (Image 3)