Secreted phosphoprotein 1 (SPP1) (AA 0-17) Antikörper

Antigen: Secreted phosphoprotein 1 (SPP1)

Synonyme: OPN, BNSP, BSPI, ETA-1, MGC110940, OP, Bsp, Eta, Opn, Ric, Opnl, Apl-1, Spp-1, AA960535, AI790405, OSP, SPP1, spp1, MGC111821

Epitope: AA 0-17

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Ratte (Rattus)

Applikation: Western Blot (WB)

Produktnummer: ABIN180183

Produktbeschreibung

Weitere Bezeichnung: Osteopontin (0-17)

Immunogen: Synthetic peptide for N-terminal of Rat Osteopontin Purification: Affinity Purified with synthetic peptide

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Osteopontin (OPN) is a secreted glycoprotein that was originally isolated from bone. Its molecular weights have been reported in the range of 66 kDa to 44 kDa depending on glycosylation and phosphorylation. OPN is also known to be expressed in other fluids and tissues including milk, urine, activated T cells, smooth muscle cells, kidney and some tumor cells. OPN contains an Arg-Gly-Asp (RGD) amino acid sequence. This motif is present in fibronectin, vitronectin and a variety of other extra-cellular proteins that bind members of the integrin family of cell surface receptors such as a vÀ 3. Recently OPN was identified as a ligand for CD44, which levels correlate with aggressiveness of lymphoid tumors and invasiveness of bladder carcinoma. Its interaction does not require RGD motif of OPN. In OPN knockout mice, it has been reported that a significantly decreased level of debridement was shown. Although the distribution and expression pattern of OPN in the human body have suggested the multiple function of OPN, its function under different situations remain obscure.

Spezifität: React to both recombinant and native rat Osteopontin Detectable to Rat Osteopontin Thrombin digested form. No crossreactivities with Human and mouse Osteopontin

Anwendungen

Applikationshinweise: This antibody can be stained in formalin fixed paraffin embedded tissues after microwave treatment. The optimal dilution is 2 ug/ml, however, the dilution rate should be optimized by each laboratories This antibody can be used for western blotting in concentration of 2 ug/ml.

Reinigung: Antigen peptide

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 . Reconstitution: 1 ml distilled water will be added to the product

Lagerung: Lyophilized product, 5 years at 2 ­ 8 C : Solution, 2 years at ­20 C

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Secreted phosphoprotein 1 (SPP1) (AA 0-17) antibody