JAB/SOCS-1 (J192) Antikörper

Antigen: JAB/SOCS-1 (J192)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Western Blot (WB)

Produktnummer: ABIN180154

Produktbeschreibung

Immunogen: Synthetic peptide for a C-terminal part of Human JAB/SOCS-1 Purification: Affinity purified with synthetic peptide

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: The Janus family of protein tyrosine kinases (JAKs) and STAT transcription factors regulate cellular processes involved in cell growth, differentiation, and transformation through their association with cytokine receptors. The CIS family of proteins (also referred as the SOCS or SSI family) has been implicated in the regulation of signal transduction by a variety of cytokines. It is reported that JAB/SOCS-1 is strongly induced by interferon-gamma and forced expression of JAB/SOCS-1 conferred cells interferon resistance. This resistance was caused by inhibition of JAK1 and JAK2 activation in response to IFN gamma. Moreover, recent detailed analysis of JAB/SOCS-1 knockout mice revealed that JAB/SOCS-1 is indeed a negative feedback regulator that determine the sensitivity of cells to IFN gamma.

Spezifität: Antigen peptide is identical among Human, Mouse and Rat

Anwendungen

Applikationshinweise: This antibody can be used for western blotting in concentration of 2 - 5 ug/ml. Cross:Antigen is used in same amino acid sequences with human, mouse and rat. Reactivity Cross reacts with mouse, but not tested cross reactivity with rat. Reference:1. Yoshimura, A. et al. A novel cytokine- inducible gene CIS encodes an SH2 containing protein that binds to tyrosine- phosphorylated interleukin 3 and erythropoietin receptors. EMBO J. 1-4: 2-816-2826, 1-995 2. Yoshimura, A. et al. The CIS/JAB family: novel negative regulators of JAK signaling pathways. Leukemia 1-2: 1-851-1857, 1-998. 3. Iwamoto T., Senga T., Naito Y., Matsuda S., Miyake Y., Yoshimura A., and Hamaguchi M. The JAK-inhibitor, JAB/SOCS-1 selectively inhibits cytokine- induced, but not v-Src induced JAK-STAT activation. Oncogene 1-9 (41), 4-795-801, 2-000

Reinigung: Antigen peptide

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 . Reconstitution: 1.0 ml distilled water will be added to the product

Lagerung: Lyophilized product, 5 years at 2 ­ 8 C : Solution, 2 years at ­20 C

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-JAB/SOCS-1 (J192) antibody