Tie-1 (9C1) Antikörper

Antigen: Tie-1 (9C1)

Klonalität: Monoklonal (9C1)

Wirt: Maus

Reaktivität: Human

Applikation: Western Blot (WB), Immunpräzipitation (IP), Durchflusszytometrie (FACS)

Produktnummer: ABIN180069

Produktbeschreibung

Immunogen: Recombinant protein of extracellular domain of human Tie-1

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 9C1

Beschreibung: TIE is a new receptor-type tyrosine kinase that was cloned from K562, a chronic myelocytic leukemia cell line. Its molecular weight is 117 kDa and its structure begins with an immunoglobulin-like domain from the extracellular N terminal, continues to 3 EGF-like domains, another immunoglobulin-like domain, 3 fibronectin III-like domains, a membrane-penetrating domain, 2 tyrosine kinase domains and a C-terminal domain. It has been reported that Tek molecules with a high homology (about 80%) have been cloned from the kinase domains. These molecules are believed to make up a Tie family. Tie-2 and a receptor type tyrosine kinase Tie-1 that is expressed specifically in endothelial cells are included in this Tie family. Among the Tie-1 gene knockout mice, a vasculature is formed in a homozygote (-/-) but the mouse is likely to succumb to pulmonary edema. Thus it has been reported that the signal via Tie-1 is important in maintaining the vascular structure. This monoclonal antibody recognizes Tie-1 by W.B. and I.P. applications and it enables FACS analysis of Tie-1-expressing cells.

Spezifität: Confirmed by western blotting with HEL (leukemia) cell.

Anwendungen

Applikationshinweise: This antibody can be used for western blotting at 1-5 ug/mL. This antibody can be used for immunoprecipitation at 3-5 ug/mL.

Reinigung: Protein A

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 . Reconstitution: 1.0 mL deionized water will be added to the product

Lagerung: Lyophilized product, 5 years at 2 ­ 8 : Solution, 2 years at ­20

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-Tie-1 (9C1) antibody

anti-Tie-1 (9C1) antibody (Image 2)

anti-Tie-1 (9C1) antibody (Image 3)