COX-2 Antikörper

Antigen: COX-2

Klonalität: Monoklonal (13H14)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (IHC), Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN180049

Produktbeschreibung

Immunogen: Synthetic peptide for a part of Human COX-2

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 13H14

Beschreibung: Cyclooxygenase (COX) is a membrane bound enzyme responsible for the oxidation of arachidonic acid to Prostaglandin G2 (PGG2) and the subsequent reduction of PGG2 to PHG2. These reactions are the first steps in the formation of a variety of prostanoids. COX has been shown to be expressed in at least two different isoforms, a constitutively expressed form, COX-1, and an inducible form, COX-2. COX-1 is thought to regulate a number of housekeeping functions, such as vascular hemostasis, renal blood flow, and maintenance of glomerular function. Inflammation mediators such as growth factors, cytokines and endotoxin induce COX-2 expression in a number of cellular systems.

Spezifität: Human COX-2 specific. No crossreactivities with Human COX-1.

Anwendungen

Applikationshinweise: This antibody can be stained in formalin fixed paraffin embedded tissues after microwave treatment by several Immunohistochemical techniques such as Avidin Biotin Complex (ABC) Method. The optimal dilution is 2-4 ug/ml, however, the dilution rate should be optimized by each laboratories. This antibody can be used for western blotting in concentration of 2-5 ug/ml. This antibody can be used for immunoprecipitation in concentration of 2-5 ug/ml.

Reinigung: Protein A

Buffer: Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 Reconstitution: 1 ml distilled water will be added to the product

Lagerung: Lyophilized product, 5 years at 2 ­ 8 : Solution, 2 years at ­20

Forschungsgebiet: Krebs

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

anti-COX-2 antibody

Human lung adenocarcinoma