IL-17 Antikörper

Antigen: IL-17

Klonalität: Monoklonal (TC11-8H4)

Wirt: Ratte

Reaktivität: Maus

Applikation: ELISA

Produktnummer: ABIN180005

Produktbeschreibung

Immunogen: E. coli-expressed, recombinant mouse IL-17

Format: Purified

Isotyp: IgG1, kappa chain  (Passende Sekundärantikörper)

Klon: TC11-8H4

Beschreibung: Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells, stimulates cell migration, cord formation, and IL-6 secretion by stromal cells CD4 memory T cells IL-17, also known as CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. Recent study has shown that IL-17 is produced by Th cells (Th17) that are distinct from the traditional Th1- and Th2-cell subsets. IL-23 plays an important role in triggering IL-17 production. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, activation of NF-kappaB and costimulation of T cell proliferation. IL-17 is an essential inflammatory mediator in the development of autoimmune diseases. Neutralization of IL-17 with monoclonal antibody is able to ameliorate the disease course. The TC11-8H4 antibody antibody reacts with mouse interleukin-17 (IL-17). Synonyms: Interleukin-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8) Structure: Cytokine, dimer, 15 kD (Mammalian)

Anwendungen

Applikationshinweise: ELISA and ELISPOT Detection: The biotinylated TC11-8H4 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified TC11-18H10 antibody (ABIN160496) as the capture antibody, and with recombinant mouse IL-17 (ABIN160666) as the standard. Additional reported applications (for the relevant formats) include: Western blotting.

Reinigung: The immunoglobulin was purified by affinity chromatography.

Buffer: Phosphate-buffered solution, pH 7.2, containing no preservative. 0.2 µm filter sterilized. Endotoxin level is < 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.

Lagerung: The antibody solution should be stored undiluted at 4 °C. This LEAF(TM) solution contains no preservative, handle under aseptic conditions.

Forschungsgebiet: Zytokine, Immunologie, Virologie, Inflammation, Krebs

Beschränkungen: Nur für Forschungszwecke einsetzbar